Autocrine induction of DNA synthesis by mechanical injury of cultured smooth muscle cells - Potential role of FGF and PDGF
| Autor: | James S. Forrester, Anna Hultgårdh-Nilsson, Sean Ameli, Bojan Cercek, Federico Calara, Jan Nilsson, Prediman K. Shah, Joel M. Kupfer, Ulf Hedin |
|---|---|
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Molecular Sequence Data Fibroblast growth factor Wounds Nonpenetrating Muscle Smooth Vascular Lesion Internal medicine medicine Animals RNA Messenger Autocrine signalling Cells Cultured Platelet-Derived Growth Factor Messenger RNA DNA synthesis biology Base Sequence Growth factor Rats Inbred Strains DNA Oligonucleotides Antisense musculoskeletal system Molecular biology Hormones Rats Fibroblast Growth Factors medicine.anatomical_structure Endocrinology Gene Expression Regulation cardiovascular system biology.protein Stress Mechanical medicine.symptom Cardiology and Cardiovascular Medicine Oligonucleotide Probes Platelet-derived growth factor receptor Blood vessel |
| Zdroj: | Karolinska Institutet Scopus-Elsevier |
| Popis: | Abstract To determine whether replication of arterial smooth muscle cells (SMCs) in response to mechanical injury would occur in the absence of serum and other cells, we created an in vitro model in which confluent, growth-arrested cultures of rat SMCs were injured by gentle pressure of a soft plastic tube and then kept in serum-free medium for up to 4 days. Replication of SMCs in and around the injury, as measured by tritiated thymidine incorporation, was noted within 24 hours and peaked at 48 hours after injury, whereas noninjured cells remained quiescent. An increased expression of platelet-derived growth factor (PDGF) A mRNA, noted 6 hours after injury, was followed by an increased PDGF AA immunoreactivity in SMCs in and around the zone of injury at 24 and 48 hours after injury. A PDGF A chain antisense oligonucleotide inhibited 87.0±4.0% ( P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|