Mice deficient for the epidermal dermokine β and γ isoforms display transient cornification defects
| Autor: | Nathalie Jonca, Anne Huchenq, Guy Serre, Emilie Leclerc, Sanja Kezic |
|---|---|
| Přispěvatelé: | Amsterdam Public Health, Coronel Institute of Occupational Health, CARBILLET, Véronique, Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Coronel Institute of Occupational Health [Amsterdam, The Netherlands] (Academic Medical Center), Amsterdam Public Health Research Institute [The Netherlands] |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Keratinocytes
Male MESH: Mice Mutant Strains Stratum corneum Filaggrin Proteins MESH: Protein Isoforms MESH: Epidermal Cells Mice [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Protein Isoforms Genetics chemistry.chemical_classification MESH: Keratinocytes / metabolism Dmkn MESH: Proteins / metabolism Cell Differentiation MESH: Cell Differentiation / physiology Phenotype Amino acid Cell biology Dermokine MESH: Proteins / genetics [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Intercellular Signaling Peptides and Proteins MESH: Animals Female Intracellular Filaggrin Gene isoform Biology MESH: Keratinocytes / cytology MESH: Mice Inbred C57BL Animals Humans MESH: Intercellular Signaling Peptides and Proteins Gene Involucrin MESH: Mice Cornified cell envelope Messenger RNA MESH: Humans Keratinocyte differentiation Proteins Cell Biology Mice Mutant Strains MESH: Male MESH: Epidermis / metabolism Mice Inbred C57BL chemistry Epidermal Cells Epidermis MESH: Female |
| Zdroj: | Journal of cell science, 127(Part 13), 2862-2872. Company of Biologists Ltd Journal of Cell Science Journal of Cell Science, 2014, 127 (13), pp.2862-2872. ⟨10.1242/jcs.144808⟩ Journal of Cell Science, Company of Biologists, 2014, 127 (13), pp.2862-2872. ⟨10.1242/jcs.144808⟩ |
| ISSN: | 0021-9533 1477-9137 |
| DOI: | 10.1242/jcs.144808⟩ |
| Popis: | International audience; Expression of the human dermokine gene (DMKN) leads to the production of four dermokine isoform families. The secreted α, β and γ isoforms have an epidermis-restricted expression pattern, with Dmkn β and γ being specifically expressed by the granular keratinocytes. The δ isoforms are intracellular and ubiquitous. Here, we performed an in-depth characterization of Dmkn expression in mouse skin and found an expression pattern that was less complex than in humans. In particular, mRNA coding for the δ family were absent. Homozygous mice null for the Dmkn β and γ isoforms had no obvious phenotype but only a temporary scaly skin during the first week of life. The pups null for the Dmkn β and γ isoforms had smaller keratohyalin granules and their cornified envelopes were more sensitive to mechanical stress. At the molecular level, amounts of profilaggrin and filaggrin monomers were reduced whereas amino acid components of the natural moisturizing factor were increased. In addition, the electrophoretic mobility of involucrin was modified, suggesting post-translational modifications. Finally, the mice null for the Dmkn β and γ isoforms strongly overexpressed Dmkn α. These data are evocative of compensatory mechanisms relevant to the temporary phenotype. Overall, we improved the knowledge of Dmkn expression in mouse and highlighted a role for Dmkn β and γ in cornification. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|