An enzyme-linked immunosorbent assay for complement regulatory proteins and membrane-bound immunoglobulins on intact red blood cells
| Autor: | H. Imrie, S. A. Rao, D. R. E. Jones, R. J. Thompson |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.drug_class
Complement receptor 1 Immunology Immunoglobulins Enzyme-Linked Immunosorbent Assay In Vitro Techniques Monoclonal antibody Complement Receptor Type 1 Antigens CD medicine Immunology and Allergy Humans Lupus Erythematosus Systemic Decay-accelerating factor Autoimmune disease Lupus erythematosus Membrane Glycoproteins biology CD55 Antigens Erythrocyte Membrane Complement System Proteins medicine.disease Immune complex Membrane protein Complement C3d Receptors Complement 3b Anemia Hemolytic Autoimmune biology.gene |
| Zdroj: | Journal of immunological methods. 177(1-2) |
| ISSN: | 0022-1759 |
| Popis: | We have developed an ELISA technique to examine RBC-bound molecules in autoimmune disorders. In particular, the technique has enabled us to investigate the hole of some complement regulatory proteins in immune complex transport and to suggest that decay accelerating factor (DAF) may be involved in this process. In both autoimmune haemolytic anaemia (AHA) and systemic lupus erythematosus (SLE) a sub-set of individuals was identified, on the basis of patterns of complement receptor 1 (CR1) expression on RBC. In these patients, CR1 identified using the monoclonal antibody E11 was low or absent whereas CR1 identified using a DAKO monoclonal antibody (C3RTo5) was present at normal levels. |
| Databáze: | OpenAIRE |
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