Reduced GLP-1 and insulin responses and glucose intolerance after gastric glucose in GRP receptor-deleted mice
| Autor: | Sonali Nayak, Ronald L. Gingerich, Keiji Wada, Bo Ahrén, Kristin Persson, Etsuko Wada |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Biology Impaired glucose tolerance Mice Glucagon-Like Peptide 1 Physiology (medical) Gastrin-releasing peptide Internal medicine Glucose Intolerance medicine Animals Insulin Protein Precursors Receptor Gastric Lavage Pancreatic hormone Mice Knockout Glucose tolerance test medicine.diagnostic_test digestive oral and skin physiology Glucagon medicine.disease Glucagon-like peptide-1 Peptide Fragments Mice Inbred C57BL Receptors Bombesin Glucose Endocrinology Knockout mouse Female hormones hormone substitutes and hormone antagonists |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 279:E956-E962 |
| ISSN: | 1522-1555 0193-1849 |
| Popis: | By applying a newly developed ELISA technique for determining biologically active intact glucagon-like peptide [GLP-1, GLP-1-(7–36)amide] in mouse, plasma baseline GLP-1 in normal NMRI mice was found to be normally distributed (4.5 ± 0.3 pmol/l; n = 72). In anesthetized mice, gastric glucose (50 or 150 mg) increased plasma GLP-1 levels two- to threefold ( P < 0.01). The simultaneous increase in plasma insulin correlated to the 10-min GLP-1 levels ( r = 0.36, P < 0.001; n = 12). C57BL/6J mice deleted of the gastrin-releasing peptide (GRP) receptor by genetic targeting had impaired glucose tolerance ( P = 0.030) and reduced early (10 min) insulin response ( P = 0.044) to gastric glucose compared with wild-type controls. Also, the GLP-1 response to gastric glucose was significantly lower in the GRP receptor-deleted mice than in the controls ( P = 0.045). In conclusion, this study has shown that 1) plasma levels of intact GLP-1 increase dose dependently on gastric glucose challenge in correlation with increased insulin levels in mice, and 2) intact GRP receptors are required for normal GLP-1 and insulin responses and glucose tolerance after gastric glucose in mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|