Cytotoxicity, Apoptosis, Cellular Uptake, Cell Cycle Distribution, and DNA-Binding Investigation of Ruthenium Complexes
| Autor: | Xin-Yan Li, Qi-Feng Guo, Qing-Hua Liu, Jian-Hua Zhao, Jian-Wei Wang, Hai-Feng Wu, Si-Hong Liu, Hui-Hua Xu |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_element
Antineoplastic Agents Apoptosis Biology Cell morphology Ruthenium chemistry.chemical_compound Bromide Cell Line Tumor Organometallic Compounds Genetics Humans Cytotoxicity Molecular Biology Biological Transport Cell Cycle Checkpoints DNA Cell Biology General Medicine Cell cycle Photochemical Processes Molecular biology chemistry Cytoplasm Nuclear chemistry |
| Zdroj: | DNA and Cell Biology. 31:1205-1213 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2011.1490 |
| Popis: | Three new ruthenium(II) polypyridyl complexes [Ru(bpy)(2)(BHIP)](2+) 1, [Ru(phen)(2)(BHIP)](2+) 2, and [Ru(dip)(2)(BHIP)](2+) 3 were synthesized and characterized. The cytotoxicity of the three complexes was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis induced by the complexes was studied by cell morphology and flow cytometry. The results showed that the percentage of apoptotic cells is 7.19%, 75.58%, and 3.51% in the presence of complexes 1, 2, and 3, respectively. The cellular uptakes were also performed and the results indicated that complexes 1, 2, and 3 can enter into the cytoplasm and also into the nucleus. The studies on antiproliferative mechanism showed the induction of S-phase arrest by complexes 1, 2, and 3. DNA-binding constants of these complexes with calf thymus DNA (CT-DNA) were determined to be 1.07 (± 0.47) × 10(5) M(-1) (s = 2.04), 1.21 (± 0.32) × 10(5) M(-1) (s = 1.88), and 2.75 (± 0.27) × 10(5) M(-1) (s = 2.17), respectively. Upon irradiation at 365 nm, complexes 1, 2, and 3 can induce cleavage of pBR322 DNA. |
| Databáze: | OpenAIRE |
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