PCR amplification of CGRP II mRNA
| Autor: | Gérard Milhaud, N. Segond, F. Lasmoles, M.S. Moukhtar, C. Calmettes, M.C. Delehaye, Stephane Minvielle, Régis Cohen, J. M. Guliana |
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| Rok vydání: | 1990 |
| Předmět: |
Calcitonin
endocrine system diseases Molecular Sequence Data Cell Oligonucleotides Thyroid Gland Biophysics Gene Expression Amplification Biology Calcitonin gene-related peptide Polymerase Chain Reaction Biochemistry Thyroid carcinoma Structural Biology Tumoral marker Gene expression Medullary thyroid carcinoma Genetics medicine Humans RNA Messenger RNA Neoplasm Thyroid Neoplasms Molecular Biology Messenger RNA Base Sequence integumentary system Carcinoma Alternative splicing Thyroid Cell Biology Molecular biology Hereditary cancer Blotting Southern medicine.anatomical_structure nervous system |
| Zdroj: | FEBS Letters. 277:243-246 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(90)80856-e |
| Popis: | Two genes code for calcitonin gene-related peptides (CGRPs). One expresses by tissue-specific alternate splicing calcitonin and CGRP I mRNAs, the other CGRP II mRNA. Calcitonin is the marker of sporadic or hereditary human medullary thyroid carcinoma (MTC). CGRP II expression is not well established in normal or tumoral thyroid. After amplification by polymerase chain reaction, CGRP I and II mRNAs were detected in six cases of MTC associated with other endocrine neoplasia (MEN IIa) and in two cases of isolated MTC. CGRP I was detected in all non-C cell tumoral thyroids (6 samples), CGRP II was barely detectable in three out of six cases. CGRP II could be a specific tumoral marker of MTC. |
| Databáze: | OpenAIRE |
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