Catalases and peroxidases histochemical detection; techniques suitable to discriminate these enzymes
| Autor: | Walter A. Hadler, Sineli R. Silveira |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Histology
Oxidative phosphorylation Iron salts chemistry.chemical_compound Bone Marrow Adrenal Glands Animals Enzyme Inhibitors Incubation chemistry.chemical_classification biology Chemistry Histocytochemistry Benzidines Cell Biology General Medicine Hydrogen Peroxide Catalase Benzidine Rats Enzyme Biochemistry Liver Peroxidases biology.protein Peroxidase |
| Zdroj: | Acta histochemica. 63(1) |
| ISSN: | 0065-1281 |
| Popis: | Summary By using the benzidine reaction, on filter paper strips loaded with catalases, peroxidases, porphyrins, haemic iron compounds and iron salts, it was possible to establish 2 histochemical techniques able to detect and discriminate catalases and peroxidases. Spot test analytical studies show that only peroxidases oxidize benzidine in presence of a 0.0015 M H 2 O 2 final concentration into the incubation medium. If a 0.0035 M H 2 O 2 final concentration is used both peroxidases and haemic iron were able to oxidize benzidine. At a 0.01 M H 2 O 2 final concentration the oxidative property of catalases become apparent and therefore at this H 2 O 2 concentration either peroxidases or haemic iron, as well as catalases could be detected. By increasing the H 2 O 2 concentration into the incubation medium, when a 4 M concentration is attained only catalases and haemic iron remain reactive. This latter H 2 O 2 concentration was chosen to detect histochemically catalases without any peroxidases interference. Using 0.0015 M and 4 M H 2 O 2 final concentrations into the incubation medium it is possible to discriminate histochemically catalases and peroxidases. Several inhibitors of catalases and peroxidases were used as an attempt to try a specific inhibition of only one of these enzymes. It was demonstrated that the use of inhibitors does not help the histochemical discrimination between catalases and peroxidases. |
| Databáze: | OpenAIRE |
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