Cilostazol Attenuates Retinal Oxidative Stress and Inflammation in a Streptozotocin-Induced Diabetic Animal Model
| Autor: | Chang-Hao Yang, Yu-Hsun Huang, Wei-Shiung Yang, Chung-May Yang, Shu-Wen Chang, Lu-Chun Wang, Po-Ting Yeh, Chung-Wu Lin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Blood Glucose
endocrine system diseases Pharmacology Phosphodiesterase 3 Inhibitors medicine.disease_cause chemistry.chemical_compound 0302 clinical medicine Chemokine CCL2 chemistry.chemical_classification Reverse Transcriptase Polymerase Chain Reaction NF-kappa B Diabetic retinopathy Intercellular Adhesion Molecule-1 Immunohistochemistry Sensory Systems Cilostazol Female medicine.symptom medicine.drug Blotting Western Enzyme-Linked Immunosorbent Assay Inflammation Retina Streptozocin Diabetes Mellitus Experimental Aqueous Humor 03 medical and health sciences Cellular and Molecular Neuroscience Diabetes mellitus medicine Animals Rats Wistar Reactive oxygen species Diabetic Retinopathy Chemokine CX3CL1 business.industry nutritional and metabolic diseases Retinal medicine.disease Streptozotocin Rats Oxidative Stress Ophthalmology Gene Expression Regulation chemistry 030221 ophthalmology & optometry Reactive Oxygen Species business 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Current Eye Research. 44:294-302 |
| ISSN: | 1460-2202 0271-3683 |
| Popis: | To investigate the anti-oxidative and anti-inflammatory effects of cilostazol in the ocular tissues of streptozotocin (STZ)-induced diabetic rats.Diabetes was induced in 6-week-old Wistar rats via peritoneal injections of STZ. The treatment group received cilostazol 18 mg/kg/day for 8 weeks (n = 10), and the diabetic group received phosphate buffer solution (n = 20). The expression of oxidative stress and inflammatory mediators in the ocular tissues was then assessed by reverse-transcription polymerase chain reactions, immunohistochemical (IHC) staining, Western blot analysis, and enzyme-linked immunosorbent assay (ELISA).Reverse-transcription polymerase chain reactions, IHC staining, Western blot analysis, and ELISA showed that cilostazol inhibited mRNA and protein expressions of intercellular adhesion molecule-1, monocyte chemoattractant protein-1 and fractalkine in the retina and aqueous humor (AqH). Consistent with these findings, cilostazol attenuated the activation of nuclear factor-κB (NF-κB) in the diabetic rats. The levels of oxidatively modified DNA (8-OHdG), nitrotyrosine and oxidative lipids (acrolein) were also diminished in the cilostazol-treated group. Chemiluminescence analysis showed that reactive oxygen species (ROS) levels in the AqH was significantly higher in the diabetic rats than in the non-diabetic rats. Treatment with cilostazol significantly reduced the ROS levels in the AqH compared to the diabetic rats.Our results indicated that cilostazol reduced inflammatory reactions and oxidative stress in diabetic eyes. The anti-inflammatory effects of cilostazol may be indirectly via reducing oxidative stress, inhibiting NF-κB activity, and subsequently decreasing inflammatory mediators. Cilostazol may be beneficial to prevent the progression of diabetic retinopathy. |
| Databáze: | OpenAIRE |
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