Lipopolysaccharides of Pectobacterium atrosepticum and Pseudomonas corrugata induce different defence response patterns in tobacco, tomato, and potato

Autor: M.-R. Barzic, S. Desender, Philippe Potin, O. Klarzynski, Florence Val, D. Andrivon
Přispěvatelé: Biologie des organismes et des populations appliquées à la protection des plantes (BIO3P), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Signaux oligosaccharidiques et lipidiques (SOL), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-GOEMAR-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST
Jazyk: angličtina
Rok vydání: 2006
Předmět:
0106 biological sciences
Lipopolysaccharides
Time Factors
Nicotiana tabacum
[SDV]Life Sciences [q-bio]
Pectobacterium
Plant Science
LIPOXYGENASE
01 natural sciences
Microbiology
03 medical and health sciences
Laminarin
chemistry.chemical_compound
Solanum lycopersicum
Species Specificity
Pseudomonas
Tobacco
SOLANACEAE
Extracellular
RELATION PLANTE-MICROORGANISME
Pectobacterium atrosepticum
Ecology
Evolution
Behavior and Systematics
Cells
Cultured
030304 developmental biology
Solanum tuberosum
0303 health sciences
biology
Dose-Response Relationship
Drug
fungi
food and beverages
General Medicine
Hydrogen Peroxide
Hydrogen-Ion Concentration
biology.organism_classification
Elicitor
Pseudomonas corrugata
POLYSACCHARIDE
chemistry
Phytophthora infestans
lipids (amino acids
peptides
and proteins)
Solanaceae
010606 plant biology & botany
Zdroj: Plant Biology
Plant Biology, Wiley, 2006, 8 (5), pp.636-645. ⟨10.1055/s-2006-924102⟩
Plant Biology, 2006, 8 (5), pp.636-645. ⟨10.1055/s-2006-924102⟩
ISSN: 1435-8603
1438-8677
DOI: 10.1055/s-2006-924102⟩
Popis: International audience; Lipopolysaccharides (LIPS), ubiquitous cell surface components of Gram-negative bacteria, are directly implicated in plant/pathogen interactions. However, their perception by the plant, the subsequent signal transduction in both compatible and incompatible interactions, as well as the defence reactions induced in compatible interactions are as yet poorly understood. We focused on biochemical and physiological reactions induced in cell suspensions of three Solanaceae species (tobacco, tomato, and potato) by purified lipopolysaccharides from Pectobacterium atrosepticum (Pa), a pathogen of potato, and Pseudomonas corrugata (Psc), a pathogen of tomato. LPSPa, and LPSPsc caused a significant acidification of potato, tomato, and tobacco extracellular media, whereas laminarin (a linear beta-1,3 oligosaccharide elicitor) induced an alkalinisation in tobacco and tomato, but not in potato cell suspensions. None of the two LIPS induced the formation of active oxygen species in any of the hosts, while laminarin induced H2O2 production in cells of tobacco but not of tomato and potato. In tomato cells, LIPSPa and LPSPsc induced a strong but transitory stimulation of lipoxygenase activity, whereas laminarin induced a stable or slightly increasing LOX activity over the first 24 h of contact. In tobacco, LOX activity was not triggered by either LPS, but significantly increased following treatment with laminarin. In potato, neither LPS nor laminarin induced LOX activity, in contrast with concentrated culture filtrate of Phytophthora infestans (CCF). These results demonstrate that LPS, as well as laminarin, are perceived in different ways by Solanaceae species, and possibly cultivars. They also suggest that defence responses modulated by LPS depend on plant genotypes rather than on the type of interaction
Databáze: OpenAIRE
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