Comet assay in human biomonitoring studies: Reliability, validation, and applications
| Autor: | Maria Dusinska, Mihalis I. Panayiotidis, Andrew Collins, Laurence Fillion, Nicholas J. Vaughan, M. F. Franklin, Katarina Raslova, Helena Petrovská, Susan J. Duthie, Martina Somorovská |
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| Rok vydání: | 1997 |
| Předmět: |
Electrophoresis
DNA Repair Epidemiology DNA repair DNA damage Health Toxicology and Mutagenesis Lymphocyte Biology Antioxidants DNA-formamidopyrimidine glycosylase Deoxyribonuclease (Pyrimidine Dimer) chemistry.chemical_compound Endonuclease Occupational Exposure Image Processing Computer-Assisted medicine Humans Lymphocytes N-Glycosyl Hydrolases Genetics (clinical) Gel electrophoresis Genetics Analysis of Variance Endodeoxyribonucleases Escherichia coli Proteins Reproducibility of Results Hydrogen Peroxide Molecular biology Comet assay Diabetes Mellitus Type 1 medicine.anatomical_structure DNA-Formamidopyrimidine Glycosylase Genetic Techniques chemistry biology.protein Oxidation-Reduction DNA DNA Damage Environmental Monitoring |
| Zdroj: | Environmental and Molecular Mutagenesis. 30:139-146 |
| ISSN: | 1098-2280 0893-6692 |
| Popis: | The comet assay (single-cell gel electrophoresis), which measures DNA strand breaks at the level of single cells, is very easily applied to human lymphocytes, and therefore lends itself to human biomonitoring studies. For the examination of DNA base oxidation (a specific marker of oxidative damage), the assay is modified by including a stage at which the DNA is incubated with a suitable lesion-specific endonuclease. Here we report on the reliability and reproducibility of this approach, from the level of comparing results from duplicate gels prepared from the same sample of cells, up to an assessment of the natural intra- and interindividual variability in lymphocyte DNA damage measured in groups of normal, healthy human volunteers. We applied the assay in investigations of human disease and occupational exposure of factory workers. |
| Databáze: | OpenAIRE |
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