Subcellular Distribution of Tail-Anchored Proteins in Arabidopsis
| Autor: | Verena Kriechbaumer, Rowena Shaw, Benjamin Abell, Anne-Marie Harrison, Caroline G. Bowsher, Joy Mukherjee |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
GTPase-activating protein
Arabidopsis Proteins Arabidopsis Cell Biology Biology Genes Plant biology.organism_classification medicine.disease_cause Biochemistry Cell biology Transmembrane domain Membrane protein Structural Biology Transcription (biology) Proteome Protein targeting Genetics medicine Humans Cloning Molecular Plastid Molecular Biology Plasmids Subcellular Fractions |
| Zdroj: | Traffic. 10:1753-1764 |
| ISSN: | 1600-0854 1398-9219 |
| DOI: | 10.1111/j.1600-0854.2009.00991.x |
| Popis: | Tail-anchored (TA) proteins function in key cellular processes in eukaryotic cells, such as vesicle trafficking, protein translocation and regulation of transcription. They anchor to internal cell membranes by a C-terminal transmembrane domain, which also serves as a targeting sequence. Targeting occurs post-translationally, via pathways that are specific to the precursor, which makes TA proteins a model system for investigating post-translational protein targeting. Bioinformatics approaches have previously been used to identify potential TA proteins in yeast and humans, yet little is known about TA proteins in plants. The identification of plant TA proteins is important for extending the post-translational model system to plastids, in addition to general proteome characterization, and the identification of functional homologues characterized in other organisms. We identified 454 loci that potentially encode TA proteins in Arabidopsis, and combined published data with new localization experiments to assign localizations to 130 proteins, including 29 associated with plastids. By analysing the tail anchor sequences of characterized proteins, we have developed a tool for predicting localization and estimate that 138 TA proteins are localized to plastids. |
| Databáze: | OpenAIRE |
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