NMR-Based Chemosensing via p-H-2 Hyperpolarization: Application to Natural Extracts
| Autor: | Nan Eshuis, Niels K. J. Hermkens, Floris P. J. T. Rutjes, Sybren S. Wijmenga, Marco Tessari, Martin C. Feiters, Bram J. A. van Weerdenburg |
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| Rok vydání: | 2016 |
| Předmět: |
Biological Products
Analyte Magnetic Resonance Spectroscopy 010405 organic chemistry Analytical chemistry chemistry.chemical_element Synthetic Organic Chemistry 010402 general chemistry Spin isomers of hydrogen 01 natural sciences Combinatorial chemistry 0104 chemical sciences Analytical Chemistry chemistry Trace analysis Hyperpolarization (physics) Iridium Biophysical Chemistry GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Physical Organic Chemistry |
| Zdroj: | Analytica Chimica Acta, 88, 3406-3412 Analytica Chimica Acta, 88, 6, pp. 3406-3412 |
| ISSN: | 0003-2670 3406-3412 |
| Popis: | When dealing with trace analysis of complex mixtures, NMR suffers from both low sensitivity and signal overlap. NMR chemosensing, in which the association between an analyte and a receptor is "signaled" by an NMR response, has been proposed as a valuable analytical tool for biofluids and natural extracts. Such chemosensors offer the possibility to simultaneously detect and distinguish different analytes in solution, which makes them particularly suitable for analytical applications on complex mixtures. In this study, we have combined NMR chemosensing with nuclear spin hyperpolarization. This was realized using an iridium complex as a receptor in the presence of parahydrogen: association of the target analytes to the metal center results in approximately 1000-fold enhancement of the NMR response. This amplification allows the detection, identification, and quantification of analytes at low-micromolar concentrations, provided they can weakly associate to the iridium chemosensor. Here, our NMR chemosensing approach was applied to the quantitative determination of several flavor components in methanol extracts of ground coffee. |
| Databáze: | OpenAIRE |
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