HLA Class I Antibodies Provoke Graft Arteriosclerosis in Human Arteries Transplanted into SCID/Beige Mice

Autor: Nassim Kamar, Lionel Rostaing, Sylvain Galvani, Mogens Thomsen, Jean-Claude Thiers, Y. Zou, Torsten Böhler, Michel Abbal, P. Stastny, Anne Nègre-Salvayre, Nathalie Augé, Cindy Canivet, Robert Salvayre, Federico Sallusto, Denis Calise
Přispěvatelé: Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Néphrologie et Transplantation d'organes [CHU Toulouse], Pôle Urologie - Néphrologie - Dialyse - Transplantations - Brûlés - Chirurgie plastique - Explorations fonctionnelles et physiologiques [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Service Médecine Interne et immunologie clinique [CHU Toulouse], Pôle Maladies de l'appareil digestif [CHU Toulouse], University of Texas Southwestern Medical Center [Dallas], Simon, Marie Francoise, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Médecine [Rangueil], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse], Department of Multiorgan Transplantation, CHU Toulouse [Toulouse], Department of Clinical Immunology
Rok vydání: 2009
Předmět:
Graft Rejection
Cellular immunity
Arteriosclerosis
[SDV]Life Sciences [q-bio]
MESH: Mesenteric Arteries
Mice
SCID
030230 surgery
Muscle
Smooth
Vascular
MESH: Histocompatibility Antigens Class I
Mice
0302 clinical medicine
Immunology and Allergy
MESH: Animals
Pharmacology (medical)
MESH: Mice
SCID
Mesenteric arteries
ComputingMilieux_MISCELLANEOUS
biology
MESH: Muscle
Smooth
Vascular
Mesenteric Arteries
3. Good health
medicine.anatomical_structure
MESH: Cell Division
Antibody
Cell Division
Blood vessel
medicine.drug_class
Transplantation
Heterologous
Antibodies
Heterophile
MESH: Graft Rejection
Human leukocyte antigen
Monoclonal antibody
03 medical and health sciences
medicine
Animals
Humans
MESH: Transplantation
Heterologous
MESH: Mice
Transplantation
MESH: Humans
business.industry
Histocompatibility Antigens Class I
MESH: Tunica Intima
medicine.disease
MESH: Arteriosclerosis
Immunology
biology.protein
Tunica Intima
business
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
MESH: Antibodies
Heterophile
030215 immunology
Zdroj: American Journal of Transplantation
American Journal of Transplantation, 2009, 9 (11), pp.2607-14. ⟨10.1111/j.1600-6143.2009.02804.x⟩
American Journal of Transplantation, Wiley, 2009, 9 (11), pp.2607-2614. ⟨10.1111/j.1600-6143.2009.02804.x⟩
American Journal of Transplantation, Wiley, 2009, 9 (11), pp.2607-14. ⟨10.1111/j.1600-6143.2009.02804.x⟩
ISSN: 1600-6135
1600-6143
DOI: 10.1111/j.1600-6143.2009.02804.x
Popis: International audience; Antibodies toward HLA class I and/or MICA are commonly observed in transplanted patients suffering from allograft arteriosclerosis, also called chronic vascular rejection (CVR). The relative importance of cellular versus humoral alloreactivity for CVR is still disputed. We demonstrate that antibodies toward HLA class I provoke lesions typical for CVR in human arteries in vivo in the absence of cellular immunity. To show this, we grafted segments of human mesenteric arteries from 8 deceased organ donors into 36 immunodeficient SCID/beige mice in the infrarenal aortic position. Three mice died postoperatively. The remaining 33 mice received weekly i.v. injections of either a monoclonal antibody toward HLA class I, toward MICA or an irrelevant monoclonal antibody. At sacrifice after 6 weeks, mice receiving the HLA antibody showed a significant neointimal thickening in the grafted artery due to smooth muscle cell (SMC) proliferation while control mice receiving anti-MICA or irrelevant antibody showed little or no thickening. Whereas antibodies toward HLA class I were mitogenic to SMC in vitro, those directed toward MICA did not have any effect. Humoral alloreactivity toward HLA may thus play a causal role for the development of CVR and this opens new possibilities for the treatment of CVR.
Databáze: OpenAIRE
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