[Production of nitric oxide metabolites during transplanted tumors growth with different metastatic potential]

Autor: N. I. Ryzhova, Deriagina Vp, L. V. Krivosheeva, I. S. Golubeva
Rok vydání: 2015
Předmět:
Zdroj: Biomeditsinskaia khimiia. 60(6)
ISSN: 2310-6972
Popis: The endogenous formation of metabolites of NO - nitrite (NI), nitrates (NA) and volatile nitrosamines in the body, tumor tissue and by abdominal cavity by macrophages for dynamics was investigated in mice F1(C57BlxCBA), Balb/c and BDF with subcutaneous transplanted tumors (Erlich carcinoma - EC and metastatic Lewis lung carcinoma - LLC). It was shown that growth of EC was accompanied by a statistically significant increase in the concentrations of NI and NA in tumor tissue to (7.3±4.67)'10-6 - (7.8±2.57)'10-5 (mol/kg) for the first three weeks and a sharp increase in urinary excretion of NI and NA. The maximum total concentration of NI and NA - (3.,6±0.46)'10-5 in tissue LLC was registered during the early stage of the tumor growth (7 days); it later declined, negatively correlating with the mass of the tumor. NI secretion by abdominal cavity macrophages demonstrated statistically significantly decrease at the stage of intensive growth LLC (14, 21 days). The tissue of EC contained varied concentration of cancerogenic N-nitrosodimethylamine and N-nitrosodiethylamine at all investigated time points. Thus, the ability of different gistogenesis tumor tissue to synthesize metabolites NO depended on time parameters and was more pronounced for EC, than LLC.V issledovaniiakh na myshakh F1(C57BlxCBA), Balb/c i BDF s podkozhno perevitymi opukholiami (kartsinoma Érlikha – KÉ i metastaziruiushchaia kartsinoma legkikh L'iuisa – KL) izuchena dinamika éndogennogo obrazovaniia metabolitov NO – nitritov (NI), nitratov (NA), letuchikh nitrozaminov v organizme, v opukholevoĭ tkani i peritoneal'nymi makrofagami. Pokazano, chto rost KÉ v techenie pervykh trekh nedel' soprovozhdaetsia statisticheski znachimym uvelicheniem summarnoĭ kontsentratsii NI+NA v opukholevoĭ tkani do urovneĭ (7,3±4,67)´10-6 – (7,8±2,57)´10-5 (mol'/kg), a takzhe rezkogo uvelicheniia vydeleniia NI+NA s mochoĭ. V to zhe vremia, v opukholevoĭ tkani KL maksimal'nyĭ uroven' kontsentratsii NI+NA (3,6±0,46kh10-5) registrirovali na 7-e sutki, v dal'neĭshem on snizhalsia, otritsatel'no korreliruia s massoĭ opukholi. Na étape intensivnogo rosta KL (14, 21 sutki) obnaruzheno dostovernoe podavlenie sekretsii NI peritoneal'nymi makrofagami. Na vsekh kontroliruemykh srokakh razvitiia opukholevaia tkan' KÉ soderzhala kantserogennye N-nitrozodimetilamin i N-nitrozodiétilamin, kontsentratsiia kotorykh sushchestvenno var'irovala. Takim obrazom, sposobnost' opukholevoĭ tkani sintezirovat' metabolity NO bolee vyrazhena u KÉ, po sravneniiu s KL i zavisit ot vremennykh parametrov rosta opukholeĭ.
Databáze: OpenAIRE