Developmental Regulation of Angiotensin Type 1 and 2 Receptor Gene Expression and Heart Growth
| Autor: | Mansel Harris, Allen D. Everett, A. Tufro-McReddie, Audrey Fisher |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class Heart growth Tetrazoles Biology Receptor Angiotensin Type 2 Losartan Receptor Angiotensin Type 1 Rats Sprague-Dawley Angiotensin Receptor Antagonists Embryonic and Fetal Development Transduction Genetic Internal medicine Renin–angiotensin system Gene expression medicine Animals Receptor Molecular Biology Messenger RNA Receptors Angiotensin Myocardium Biphenyl Compounds Hemodynamics Imidazoles Gene Expression Regulation Developmental Heart Receptor antagonist Angiotensin II Rats Endocrinology Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Journal of Molecular and Cellular Cardiology. 29:141-148 |
| ISSN: | 0022-2828 |
| DOI: | 10.1006/jmcc.1996.0259 |
| Popis: | Little is known regarding the developmental regulation of the cardiac angiotensin type 1 (AT 1 ) and type 2 (AT 2 ) receptor genes or their role in normal cardiac growth. Regulation of AT 1 and AT 2 receptor genes were examined using total and poly A+RNA isolated from whole Sprague–Dawley rat hearts. AT 1 mRNA levels were 3.5-fold higher in the 19-day-old fetal heart compared to the 90-day-old adult as detected with 2 or 5 μ g of poly A+RNA. AT 2 mRNA was only detectable with 20 μ g of poly A+RNA. AT 2 mRNA levels were highest in the 19-day-old fetal heart with no detectable message in the 90-day-old adult heart. Qualitative PCR for AT 2 mRNA also could not detect AT 2 mRNA in the adult heart. Treatment with the AT 1 receptor antagonist losartan for 3 weeks in the 21-day-old rat or for 4 days in the 38-day-old rat resulted in a significant decrease in heart/body weight in both groups and body weight in the 3-week treatment group. AT 2 blockade for 4 days with PD123319 or β -receptor blockade with propranolol for 3 weeks did not alter heart/body weights. Losartan treatment also resulted in a three-fold increase in cardiac AT 1 mRNA levels in both the 4-day and 3-week treatment groups compared to controls. We conclude that Ang II, acting primarily, if not exclusively via the AT 1 receptor plays a significant role in the regulation of normal cardiac growth in the young rat. |
| Databáze: | OpenAIRE |
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