Multiple leader sequences for mouse cathepsin B mRNA?
| Autor: | Daniel Béchet, H. Rhaissi, Marc Ferrara |
|---|---|
| Přispěvatelé: | Station de recherches sur la viande, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Rok vydání: | 1993 |
| Předmět: |
DNA
Complementary Transcription Genetic Molecular Sequence Data Biology Polymerase Chain Reaction Biochemistry Cathepsin B Mice 03 medical and health sciences Exon Eukaryotic translation Rapid amplification of cDNA ends [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Cloning Molecular Promoter Regions Genetic Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences Base Sequence 030302 biochemistry & molecular biology Nucleic acid sequence Intron Promoter 3T3 Cells Exons General Medicine Molecular biology Introns CLONAGE DE GENE Kirsten murine sarcoma virus |
| Zdroj: | Biochimie Biochimie, Elsevier, 1993, 75, pp.899-904 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/0300-9084(93)90046-u |
| Popis: | We previously described the gene structure of murine cathepsin B. OUr results suggested that the 5′-untranslated region (leader) is interrupted by a large intron. The second exon (exon 2) contains the translation initiation site. To characterize the leader region, a rapid amplification of cDNA ends (RACE) procedure was developed. The PCR products were directly cloned and sequenced. Nucleotide sequence analyses revealed three different 5′-cDNA ends, suggesting the existence of three different leader regions. In addition to the leader (LA) previously characterized, we now describe two other 5′-untranslated regions, LB and LC. Leader LB is located 2.3 kb upstream exon-2, and leader LC corresponds to the 3′-end of the first intron and is thus contiguous to exon-2. Our results suggest for murine cathepsin B gene the presence of multiple promoters, and possibly the expression of multiple mRNAs differing in their leader region. |
| Databáze: | OpenAIRE |
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