De novo transcriptome analysis of the mussel Perna viridis after exposure to the toxic dinoflagellate Prorocentrum lima
| Autor: | Hong-Ye Li, Jie-Sheng Liu, Jian-Wei Zheng, Min Dou, Gong Zhang, Yu-hu Jiao, Wei-Dong Yang |
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| Rok vydání: | 2019 |
| Předmět: |
Perna
NF-E2-Related Factor 2 Health Toxicology and Mutagenesis 0211 other engineering and technologies Defence mechanisms Down-Regulation Gene Expression ATP-binding cassette transporter 02 engineering and technology 010501 environmental sciences Real-Time Polymerase Chain Reaction 01 natural sciences Microbiology Transcriptome chemistry.chemical_compound Immune system stomatognathic system Animals Shellfish Poisoning 0105 earth and related environmental sciences 021110 strategic defence & security studies biology Gene Expression Profiling Public Health Environmental and Occupational Health Dinoflagellate General Medicine Mussel Okadaic acid biology.organism_classification Pollution Up-Regulation chemistry Seafood Dinoflagellida Marine Toxins Perna viridis |
| Zdroj: | Ecotoxicology and environmental safety. 192 |
| ISSN: | 1090-2414 |
| Popis: | Diarrheic shellfish poisoning (DSP) toxins are produced by harmful microalgae and accumulate in bivalve mollusks, causing various toxicity. These toxic effects appear to abate with increasing DSP concentration and longer exposure time, however, the underlying mechanisms remain unclear. To explore the underlying molecular mechanisms, de novo transcriptome analysis of the digestive gland of Perna viridis was performed after Prorocentrum lima exposure. RNA-seq analysis showed that 1886 and 237 genes were up- and down-regulated, respectively after 6 h exposure to P. lima, while 265 genes were up-regulated and 217 genes were down-regulated after 96 h compared to the control. These differentially expressed genes mainly involved in Nrf2 signing pathways, immune stress, apoptosis and cytoskeleton, etc. Combined with qPCR results, we speculated that the mussel P. viridis might mainly rely on glutathione S-transferase (GST) and ABC transporters to counteract DSP toxins during short-term exposure. However, longer exposure of P. lima could activate the Nrf2 signaling pathway and inhibitors of apoptosis protein (IAP), which in turn reduced the damage of DSP toxins to the mussel. DSP toxins could induce cytoskeleton destabilization and had some negative impact on the immune system of bivalves. Collectively, our findings uncovered the crucial molecular mechanisms and the regulatory metabolic nodes that underpin the defense mechanism of bivalves against DSP toxins and also advanced our current understanding of bivalve defense mechanisms. |
| Databáze: | OpenAIRE |
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