Elevated prolactin redirects secretory vesicle traffic in rabbit lacrimal acinar cells
| Autor: | C.T. Chiu, T. Nakamura, Ameae M. Walker, Barbara Petridou, Austin K. Mircheff, Sarah F. Hamm-Alvarez, Yanru Wang, Melvin D. Trousdale, Joel E. Schechter |
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| Přispěvatelé: | University of Southern California (USC), Division of Biomedical Sciences, St. George’s Hospital University, Unité de recherche génomique et physiologie de la lactation (GPL), Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2006 |
| Předmět: |
Physiology
Endocrinology Diabetes and Metabolism rab3 GTP-Binding Proteins LACRIMAR ACINAR CELLS OCULAR SURFACE MUCOSAL IMMUNITY PREGNANCY SJOGREN'S SYNDROME oryctolagus cuniculus 0302 clinical medicine Acinus Transduction Genetic ComputingMilieux_MISCELLANEOUS Cells Cultured Cytoskeleton 0303 health sciences Lacrimal Apparatus [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Secretory Vesicle beta-N-Acetylhexosaminidases Protein Transport medicine.anatomical_structure Endocrinologie et métabolisme Female Rabbits endocrine system medicine.medical_specialty Recombinant Fusion Proteins Genetic Vectors Green Fluorescent Proteins Biology Lacrimal apparatus Exocytosis Adenoviridae 03 medical and health sciences gestation Physiology (medical) Internal medicine medicine Acinar cell Animals Secretion lapin RNA Messenger Autocrine signalling 030304 developmental biology Endocrinology and metabolism Secretory Vesicles Membrane Proteins Biological Transport Intracellular Membranes Prolactin Endocrinology Secretory protein 030221 ophthalmology & optometry glande lacrimale Carbachol Carrier Proteins prolactine |
| Zdroj: | AJP-Endocrinology and Metabolism AJP-Endocrinology and Metabolism, American Physiological Society, 2007, 292 (4), pp.E1122-E1134. ⟨10.1152/ajpendo.00381.2006⟩ American Journal of Physiology. Endocrinology and Metabolism 4 (292), E1122-E1134. (2007) |
| ISSN: | 0193-1849 1522-1555 |
| DOI: | 10.1152/ajpendo.00381.2006⟩ |
| Popis: | During pregnancy, lymphocytes infiltrating the rabbit lacrimal gland disperse to the interacinar space from their normal focal concentrations, basal fluid secretion decreases, pilocarpine-induced fluid secretion increases, and stimulated fluid protein concentration decreases. Ductal epithelial cell prolactin (PRL) content increases and redistributes from the apical to the basal-lateral cytoplasm. A replication-incompetent adenovirus vector for rabbit PRL (AdPRL) was used to test the hypothesis that increased intracrine/autocrine PRL signaling alters secretory protein traffic in an ex vivo lacrimal acinar cell model. AdPRL had no discernable influence on microtubules or actin microfilaments or their responses to carbachol (CCh). Endogenous and transduced PRLs exhibited similar, nonpolarized, punctate distributions. Cells secreted PRL consititutively and at increased rates in response to CCh. In contrast, constitutive secretion of β-hexosaminidase was negligible, suggesting that the constitutive pathway for PRL is relatively inaccessible to typical secretory proteins. AdPRL had no significant effect on total secretion of β-hexosaminidase or syncollin-green fluorescent protein (GFP), a chimeric secretory protein construct. However, it reversed the polarized distributions of vesicles containing rab3D and syncollin-GFP. Live-cell imaging indicated that AdPRL redirected CCh-dependent syncollin-GFP exocytosis from the apical plasma membrane to the basal-lateral membrane. Elevated concentrations of exogenous rabbit PRL in the ambient medium elicited similar changes. These observations suggest that elevated PRL, as occurs in the physiological hyperprolactinemia of pregnancy, induces lacrimal epithelial cells to express a mixed exocrine/endocrine phenotype that secretes fluid to the acinus-duct lumen but secretes proteins to the underlying tissue space. This phenotype may contribute to the pregnancy-associated immunoarchitecture. |
| Databáze: | OpenAIRE |
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