Combined microRNA and mRNA detection in mammalian retinas by in situ hybridization chain reaction
| Autor: | Ryan M. Welchko, Robert C. Thompson, Shunbin Xu, Huanqing Zhang, Pei Zhuang, David L. Turner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
In situ lcsh:Medicine In situ hybridization Article Retina Mice 03 medical and health sciences 0302 clinical medicine microRNA medicine Animals RNA Messenger lcsh:Science In Situ Hybridization Regulation of gene expression Messenger RNA Multidisciplinary Chemistry lcsh:R Cell type diversity RNA 3. Good health Cell biology MicroRNAs Amacrine Cells 030104 developmental biology medicine.anatomical_structure miRNAs lcsh:Q human activities 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Improved in situ hybridization methods for mRNA detection in tissues have been developed based on the hybridization chain reaction (HCR). We show that in situ HCR methods can be used for the detection of microRNAs in tissue sections from mouse retinas. In situ HCR can be used for the detection of two microRNAs simultaneously or for the combined detection of microRNA and mRNA. In addition, miRNA in situ HCR can be combined with immunodetection of proteins. We use these methods to characterize cells expressing specific microRNAs in the mouse retina. We find that miR-181a is expressed in amacrine cells during development and in adult retinas, and it is present in both GABAergic and glycinergic amacrine cells. The detection of microRNAs with in situ HCR should facilitate studies of microRNA function and gene regulation in the retina and other tissues. |
| Databáze: | OpenAIRE |
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