Absence of human T-lymphotropic virus type I tax sequences in a population of normal blood donors in the Baltimore, MD/Washington, DC, area: results from a multicenter study
| Autor: | B A Pancake, Alan E. Williams, A Vallejo, Renu B. Lal, R K Alexander, Charlene S. Dezzutti, Edward Tabor, D Gallahan, Indira Hewlett, Paul R. McCurdy, Elliot P. Cowan, D Zucker-Franklin, K George, George J. Nemo |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Sequence analysis viruses Immunology Population Blood Donors Human T-lymphotropic virus Peripheral blood mononuclear cell Virus Immunoenzyme Techniques immune system diseases hemic and lymphatic diseases HIV Seronegativity HIV Seropositivity Immunology and Allergy Medicine Humans Mass Screening Multicenter Studies as Topic education Mass screening Aged education.field_of_study Human T-lymphotropic virus 1 Deltaretrovirus Infections biology business.industry Deltaretrovirus Antibodies Genes pX virus diseases Hematology Sequence Analysis DNA Middle Aged biology.organism_classification Virology Baltimore District of Columbia biology.protein Female Viral disease Antibody business |
| Zdroj: | Transfusion. 39(8) |
| ISSN: | 0041-1132 |
| Popis: | BACKGROUND: It was reported recently that sequences corresponding to the human T-lymphotropic virus type I (HTLV-I) tax gene were detected in peripheral blood mononuclear cells from 8 to 11 percent of healthy blood donors without detectable antibodies to HTLV-I. A multicenter blind study was conducted to determine if these results could be independently confirmed. STUDY DESIGN AND METHODS: Specimens were collected from 100 anti-HTLV-I-negative healthy blood donors and from 11 anti-HTLV-I- or anti-HTLV-II-positive individuals. All samples were coded and distributed to each of four independent testing laboratories for polymerase chain reaction analysis to detect sequences of the HTLV-I or HTLV-II tax gene, using detailed procedures specified by the laboratory reporting the original observation. Each laboratory also tested a dilution panel of a plasmid containing HTLV-I tax to determine the analytical sensitivity of the procedure. RESULTS: The analytical sensitivity of the screening methods permitted detection of as few as 1 to 10 copies of the tax gene. However, HTLV-I tax sequences could not be detected in any of the anti-HTLV-I-negative blood donors at more than one test site. CONCLUSION: HTLV-I tax sequences appear not to be present in this population of 100 blood donors negative for anti-HTLV-I. |
| Databáze: | OpenAIRE |
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