Combining retinal-based and chlorophyll-based (oxygenic) photosynthesis: Proteorhodopsin expression increases growth rate and fitness of a ∆PSI strain of Synechocystis sp. PCC6803
Autor: | Otilia Cheregi, Srividya Ganapathy, Christiane Funk, Jos C. Arents, Willem J. de Grip, Klaas J. Hellingwerf, J. Merijn Schuurmans, Filipe Branco dos Santos, Que Chen |
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Přispěvatelé: | Molecular Microbial Physiology (SILS, FNWI), Freshwater and Marine Ecology (IBED, FNWI) |
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
Chlorophyll Light Bioengineering Photosynthesis 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound All institutes and research themes of the Radboud University Medical Center 010608 biotechnology Rhodopsins Microbial Escherichia coli Growth rate 030304 developmental biology 0303 health sciences Proteorhodopsin biology Strain (chemistry) Synechocystis Oxygen evolution NADPH Dehydrogenase Retinal biology.organism_classification Culture Media Oxygen Glucose chemistry Conjugation Genetic biology.protein Biophysics Retinaldehyde High Energy Physics::Experiment Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19] Biotechnology |
Zdroj: | Metabolic Engineering, 52, 68-76 Metabolic Engineering, 52, pp. 68-76 Metabolic Engineering, 52, 68-76. Academic Press Inc. |
ISSN: | 1096-7176 |
Popis: | To fill the “green absorption gap”, a green absorbing proteorhodopsin was expressed in a PSI-deletion strain (ΔPSI) of Synechocystis sp. PCC6803. Growth-rate measurements, competition experiments and physiological characterization of the proteorhodopsin-expressing strains, relative to the ΔPSI control strain, allow us to conclude that proteorhodopsin can enhance the rate of photoheterotrophic growth of ΔPSI Synechocystis strain. The physiological characterization included measurement of the amount of residual glucose in the spent medium and analysis of oxygen uptake- and production rates. To explore the use of solar radiation beyond the PAR region, a red-shifted variant Proteorhodopsin-D212N/F234S was expressed in a retinal-deficient PSI-deletion strain (ΔPSI/ΔSynACO). Via exogenous addition of retinal analogue an infrared absorbing pigment (maximally at 740 nm) was reconstituted in vivo. However, upon illumination with 746 nm light, it did not significantly stimulate the growth (rate) of this mutant. The inability of the proteorhodopsin-expressing ΔPSI strain to grow photoautotrophically is most likely due to a kinetic rather than a thermodynamic limitation of its NADPH-dehydrogenase in NADP+-reduction. |
Databáze: | OpenAIRE |
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