| Autor: |
Sisakova, Alexandra, Altmannova, Veronika, Sebesta, Marek, Krejci, Lumir |
| Rok vydání: |
2017 |
| DOI: |
10.6084/m9.figshare.c.3894763_d4 |
| Popis: |
Stimulation of Mus81-Mms4 nuclease activity by RFC complex. (A) RFC stimulation of human MUS81-EME1 complex. Reaction mixtures containing DNA substrate (5 nM) and the Mus81-Mms4 (0.25 nM, lanes 1–5) or MUS81-EME1 (0.25 nM, lanes 6–10) were incubated with increasing amounts of RFC complex (0.25, 0.5, 1.25, and 2.5 nM) at 37 °C for 20 min and then analyzed. (B–G) Time course enhancement of the Mus81 complex nuclease activity by RFC on various DNA substrates. Mus81-Mms4 (0.2 nM) was incubated with indicated DNA substrates (4 nM) in the presence or absence of RFC (2 nM). Reactions were incubated at 37 °C for 60 min. Aliquots of the reactions were taken at the indicated times and analyzed. Quantification of data from three independent experiments was performed for each DNA substrate. *Raw data provided in Additional file 2. (PDF 2850 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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