Ribonucleotide Reductase: Evidence for Specific Association with HeLa-Cell Mitochondria
| Autor: | Christopher K. Mathews, J M Leeds, M B Slabaugh, Patrick Young |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cytoplasm
Ribonucleoside Diphosphate Reductase Immunoblotting Cell Biophysics Stimulation Reductase Mitochondrion Cell Fractionation Biochemistry HeLa chemistry.chemical_compound Ribonucleotide Reductases Centrifugation Density Gradient medicine Humans Molecular Biology biology Cell Biology biology.organism_classification Molecular biology Enzyme assay Mitochondria Deoxyribonucleoside Kinetics medicine.anatomical_structure Ribonucleotide reductase chemistry biology.protein HeLa Cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 203:46-52 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.2146 |
| Popis: | Mammalian mitochondria contain pools of deoxyribonucleoside 5′-triphosphates that behave differently from the much larger whole-cell pools. To investigate the origins of these pools, we analyzed HeLa cell mitochondria for ribonucleotide reductase activity. Three findings suggest specific association of a reductase with mitochondria: (1) enzyme activity in extracts of washed mitochondria, (2) stimulation of that activity by dATP at levels inhibitory to the major cellular activity, and (3) association of immunoreactive material with washed and fractionated mitochondria. |
| Databáze: | OpenAIRE |
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