| Autor: |
Yu-Fei Ao, Hui-Juan Hu, De-Xian Wang, Qi-Qiang Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Green Synthesis and Catalysis, Vol 2, Iss 3, Pp 324-327 (2021) |
| ISSN: |
2666-5549 |
| Popis: |
A versatile biocatalytic desymmetric method for efficiently accessing enantiopure cis-3,4-disubstituted pyrrolidines was developed. Catalyzed by amidase-containing E. coli whole cells, a series of meso pyrrolidine-2,5-dicarboxamides were hydrolyzed to obtain 4-carbamoylpyrrolidine-3-carboxylic acid derivatives in 47%–95% yields and 62% ∼ >99.5% ee values under mild condition. The catalytic efficiency and enantioselectivity are related to the substituents in the phenyl ring. The enzyme-substrate binding mode is established and the high enantioselectivity of amidase is revealed by MD simulations. The improvement of biocatalytic efficiency has been preliminarily explored through protein engineering. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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