Community Laboratory Testing for Cryptosporidium: Multicenter Study Retesting Public Health Surveillance Stool Samples Positive for Cryptosporidium by Rapid Cartridge Assay with Direct Fluorescent Antibody Testing

Autor: Susan Madison-Antenucci, Shari Shea, Tam T. Van, Dave Boxrud, Jonathan S. Yoder, Sarah A. Collier, Michael J. Beach, Kirsten Larson, Leigh Ann Bates, Trisha J. Robinson, Lihua Xiao, Timothy Monson, Dawn M. Roellig, Anna J. Blackstock
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
Cryptosporidiosis
lcsh:Medicine
Geographical locations
Feces
0302 clinical medicine
Public health surveillance
Specimen Storage
Epidemiology
Medicine and Health Sciences
Public and Occupational Health
Public Health Surveillance
030212 general & internal medicine
Child
lcsh:Science
Protozoans
Disease surveillance
Multidisciplinary
biology
Cryptosporidium
Middle Aged
3. Good health
Chemistry
Fluorescent Antibody Technique
Direct
Child
Preschool
Physical Sciences
Biological Assay
Female
Sample collection
Research Article
Adult
medicine.medical_specialty
Adolescent
Minnesota
030231 tropical medicine
Antigens
Protozoan
Research and Analysis Methods
03 medical and health sciences
Young Adult
Wisconsin
Internal medicine
medicine
Parasitic Diseases
Humans
Direct fluorescent antibody
business.industry
Clinical Laboratory Techniques
Public health
lcsh:R
Organisms
Chemical Compounds
Infant
Newborn
Outbreak
Biology and Life Sciences
Infant
biology.organism_classification
Parasitic Protozoans
United States
Storage and Handling
Immunology
North America
lcsh:Q
People and places
business
Preservatives
Zdroj: PLoS ONE, Vol 12, Iss 1, p e0169915 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Cryptosporidium is a common cause of sporadic diarrheal disease and outbreaks in the United States. Increasingly, immunochromatography-based rapid cartridge assays (RCAs) are providing community laboratories with a quick cryptosporidiosis diagnostic method. In the current study, the Centers for Disease Control and Prevention (CDC), the Association of Public Health Laboratories (APHL), and four state health departments evaluated RCA-positive samples obtained during routine Cryptosporidium testing. All samples underwent "head to head" re-testing using both RCA and direct fluorescence assay (DFA). Community level results from three sites indicated that 54.4% (166/305) of Meridian ImmunoCard STAT! positives and 87.0% (67/77) of Remel Xpect positives were confirmed by DFA. When samples were retested by RCA at state laboratories and compared with DFA, 83.3% (155/186) of Meridian ImmunoCard STAT! positives and 95.2% (60/63) of Remel Xpect positives were confirmed. The percentage of confirmed community results varied by site: Minnesota, 39.0%; New York, 63.9%; and Wisconsin, 72.1%. The percentage of confirmed community results decreased with patient age; 12.5% of community positive tests could be confirmed by DFA for patients 60 years of age or older. The percentage of confirmed results did not differ significantly by sex, storage temperature, time between sample collection and testing, or season. Findings from this study demonstrate a lower confirmation rate of community RCA positives when compared to RCA positives identified at state laboratories. Elucidating the causes of decreased test performance in order to improve overall community laboratory performance of these tests is critical for understanding the epidemiology of cryptosporidiosis in the United States (US).
Databáze: OpenAIRE
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