cAMP controls a trafficking mechanism that maintains the neuron specificity and subcellular placement of electrical synapses during development
Autor: | Sydney Heifner, Stephen E Von Stetina, Isaiah Swann, Sierra Palumbos, Rebecca McWhirter, Amanda C. Mitchell, David M. Miller, Rachel L. Skelton |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Nervous system
Biology Gap junction assembly General Biochemistry Genetics and Molecular Biology Article Connexins Animals Genetically Modified Electrical Synapses Cyclic AMP medicine Animals Electrical synapse Axon Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Homeodomain Proteins Motor Neurons Neurons Gap junction Gap Junctions Cell Biology Axons medicine.anatomical_structure Gene Expression Regulation nervous system Synapses Soma Neuron Neuroscience Subcellular Fractions Developmental Biology |
Zdroj: | Dev Cell |
Popis: | Summary Electrical synapses are established between specific neurons and within distinct subcellular compartments, but the mechanisms that direct gap junction assembly in the nervous system are largely unknown. Here, we show that a developmental program tunes cAMP signaling to direct the neuron-specific assembly and placement of electrical synapses in the C. elegans motor circuit. We use live-cell imaging to visualize electrical synapses in vivo and an optogenetic assay to confirm that they are functional. In ventral A class (VA) motor neurons, the UNC-4 transcription factor blocks expression of cAMP antagonists that promote gap junction miswiring. In unc-4 mutants, VA electrical synapses are established with an alternative synaptic partner and are repositioned from the VA axon to soma. cAMP counters these effects by driving gap junction trafficking into the VA axon for electrical synapse assembly. Thus, our experiments establish that cAMP regulates gap junction trafficking for the biogenesis of functional electrical synapses. |
Databáze: | OpenAIRE |
Externí odkaz: |