Spinal P2X7R contributes to streptozotocin-induced mechanical allodynia in mice
| Autor: | Xiang Xu, Cheng-Ming Ni, Hui Jin, Bing-Yu Ling, Hong Cao, Zhi-Qi Zhao, He-Ping Sun, Yu-Qiu Zhang, Lan Xu |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Diabetic neuropathy Streptozocin Article General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Mice 03 medical and health sciences 0302 clinical medicine Diabetic Neuropathies Internal medicine Diabetes mellitus Acetamides medicine Animals General Pharmacology Toxicology and Pharmaceutics General Veterinary Microglia business.industry General Medicine Streptozotocin Spinal cord medicine.disease Mice Inbred C57BL Lumbar Spinal Cord Diabetes Mellitus Type 1 030104 developmental biology medicine.anatomical_structure Endocrinology Peripheral neuropathy Spinal Cord Hyperalgesia 030220 oncology & carcinogenesis Neuropathic pain Quinolines Receptors Purinergic P2X7 business medicine.drug |
| Zdroj: | Journal of Zhejiang University-SCIENCE B. 21:155-165 |
| ISSN: | 1862-1783 1673-1581 |
| Popis: | Painful diabetic neuropathy (PDN) is a diabetes mellitus complication. Unfortunately, the mechanisms underlying PDN are still poorly understood. Adenosine triphosphate (ATP)-gated P2X7 receptor (P2X7R) plays a pivotal role in non-diabetic neuropathic pain, but little is known about its effects on streptozotocin (STZ)-induced peripheral neuropathy. Here, we explored whether spinal cord P2X7R was correlated with the generation of mechanical allodynia (MA) in STZ-induced type 1 diabetic neuropathy in mice. MA was assessed by measuring paw withdrawal thresholds and western blotting. Immunohistochemistry was applied to analyze the protein expression levels and localization of P2X7R. STZ-induced mice expressed increased P2X7R in the dorsal horn of the lumbar spinal cord during MA. Mice injected intrathecally with a selective antagonist of P2X7R and P2X7R knockout (KO) mice both presented attenuated progression of MA. Double-immunofluorescent labeling demonstrated that P2X7R-positive cells were mostly co-expressed with Iba1 (a microglia marker). Our results suggest that P2X7R plays an important role in the development of MA and could be used as a cellular target for treating PDN. |
| Databáze: | OpenAIRE |
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