Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures
| Autor: | Richard Weiss, Sara Michelini, Ancuela Andosch, Jutta Horejs-Hoeck, Albert Duschl, Damjana Drobne, Thomas Verwanger, Philip Steiner, Víctor F. Puntes, Francesco Barbero, Ursula Lütz-Meindl, Alessandra Prinelli |
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| Přispěvatelé: | European Commission, Austrian Science Fund |
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
CD86 0303 health sciences Lipopolysaccharide Endosome Chemistry Effector Metal Nanoparticles Dendritic Cells 02 engineering and technology Dendritic cell 021001 nanoscience & nanotechnology Phenotype Cell biology 03 medical and health sciences chemistry.chemical_compound Immune system Downregulation and upregulation Humans General Materials Science Gold 0210 nano-technology 030304 developmental biology |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Digital.CSIC. Repositorio Institucional del CSIC instname Nanoscale |
| Popis: | Dendritic cells (DCs) shape immune responses by influencing T-cell activation. Thus, they are considered both an interesting model for studying nano-immune interactions and a promising target for nano-based biomedical applications. However, the accentuated ability of nanoparticles (NPs) to interact with biomolecules may have an impact on DC function that poses an unexpected risk of unbalanced immune reactions. Here, we investigated the potential effects of gold nanoparticles (AuNPs) on DC function and the consequences for effector and memory T-cell responses in the presence of the microbial inflammatory stimulus lipopolysaccharide (LPS). Overall, we found that, in the absence of LPS, none of the tested NPs induced a DC response. However, whereas 4-, 8-, and 11 nm AuNPs did not modulate LPS-dependent immune responses, 26 nm AuNPs shifted the phenotype of LPS-activated DCs toward a tolerogenic state, characterized by downregulation of CD86, IL-12 and IL-27, upregulation of ILT3, and induction of class E compartments. Moreover, this DC phenotype was less proficient in promoting Th1 activation and central memory T-cell proliferation. Taken together, these findings support the perception that AuNPs are safe under homeostatic conditions; however, particular care should be taken in patients experiencing a current infection or disorders of the immune system. This study shows that gold nanoparticles promote the differentiation of dendritic cells to a tolerogenic-like phenotype, affecting their ability to induce antibacterial immune responses mediated by Th1 cells and to activate central memory T cells. |
| Databáze: | OpenAIRE |
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