Cardiac Fibroblasts Recruit Th17 Cells Infiltration Into Myocardium by Secreting CCL20 in CVB3-Induced acute Viral Myocarditis
Autor: | Ming-Xin Zhu, Wei Liang, Miao Yu, Jun Hu, Tong Zhao, Xiang Cheng, Jing Yuan, Shuang Wen, He-Ping Guo, Qi Long, Yuhua Liao, Huan-Huan Li, Min Wang |
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Rok vydání: | 2013 |
Předmět: |
Male
Pathology medicine.medical_specialty Viral Myocarditis Endothelium Physiology Cellular differentiation Cell Coxsackievirus Infections chemical and pharmacologic phenomena lcsh:Physiology lcsh:Biochemistry Mice medicine Animals Humans Myocyte Myocytes Cardiac lcsh:QD415-436 Th17 cells Mice Inbred BALB C Chemokine CCL20 Cardiac fibroblasts Acute viral myocarditis lcsh:QP1-981 business.industry Myocardium Cell Differentiation Heart hemic and immune systems Cell migration Fibroblasts respiratory system medicine.disease Enterovirus B Human CCL20 Myocarditis medicine.anatomical_structure Acute Disease Cancer research business Infiltration (medical) |
Zdroj: | Cellular Physiology and Biochemistry, Vol 32, Iss 5, Pp 1437-1450 (2013) |
ISSN: | 1421-9778 1015-8987 |
Popis: | Aims: Th17 cells contributed to myocardial inflammatory injury in acute viral myocarditis (AVMC), and the migration of these cells were mainly mediated by CCL20-secreting inflammatory cells. However, whether and how the resident cells such as cardiomyocytes and cardiac fibroblasts could mediate Th17 cell migration into the heart remains unclear in AVMC. Methods: The effect of CCL20 on the dynamic alterations of intracardiac Th17 cells and disease severity were investigated through the neutralization of CCL20 in AVMC mice. The key cells releasing CCL20 in the heart and the effects of CCL20-secreting cells on Th17 cell arrest, migration and differentiation were detected in vitro. Results: Neutralization of CCL20 efficiently repressed the myocardial inflammation along with the reduction of Th17 cell infiltrations in the course of AVMC. In vitro, after stimulations of TNF-α, IL-1β and IL-17, cardiac fibroblasts rather than cardiomyocytes could be dominantly induced for CCL20 production. CCL20-secreting cardiac fibroblasts boosted Th17 cell arrest on endothelium, and induce Th17 cell migration. However, CCL20 produced by cardiac fibroblasts had no effect on Th17 cell differentiation and IL-17 production. Conclusions: It firstly suggested that cardiac fibroblasts could recruit Th17 cells infiltration into myocardium by secreting CCL20 in AVMC. |
Databáze: | OpenAIRE |
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