CRISPR/Cas9 gene editing in a chicken model: current approaches and applications
| Autor: | Luiza Chojnacka-Puchta, Dorota Sawicka |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Computational biology Biology Animal Genetics • Review Genome engineering 03 medical and health sciences 0302 clinical medicine Genome editing Genetics Animals Humans CRISPR Primordial germ cells Egg Hypersensitivity Gene Subgenomic mRNA Gene Editing Genome Cas9 CRISPR/Cas9 system General Medicine Chicken Disease Models Animal Germ Cells Phenotype 030104 developmental biology Gesicle technology CRISPR-Cas Systems Homologous recombination Chickens 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | Journal of Applied Genetics |
| ISSN: | 2190-3883 1234-1983 |
| Popis: | Improvements in genome editing technology in birds using primordial germ cells (PGCs) have made the development of innovative era genome-edited avian models possible, including specific chicken bioreactors, production of knock-in/out chickens, low-allergenicity eggs, and disease-resistance models. New strategies, including CRISPR/Cas9, have made gene editing easy and highly efficient in comparison to the well-known process of homologous recombination. The clustered regularly interspaced short palindromic repeats (CRISPR) technique enables us to understand the function of genes and/or to modify the animal phenotype to fit a specific scientific or production target. To facilitate chicken genome engineering applications, we present a concise description of the method and current application of the CRISPR/Cas9 system in chickens. Different strategies for delivering sgRNAs and the Cas9 protein, we also present extensively. Furthermore, we describe a new gesicle technology as a way to deliver Cas9/sgRNA complexes into target cells, and we discuss the advantages and describe basal applications of the CRISPR/Cas9 system in a chicken model. |
| Databáze: | OpenAIRE |
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