Adipogenic function of mouse tetranectin and identification of its functional domain
| Autor: | Jihoe Kim, Da-young Ryu, Jihyun Park, Safikur Rahman |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Plasmin Biophysics Serum protein Biochemistry law.invention 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine law Adipocyte 3T3-L1 Cells medicine Adipocytes Animals Lectins C-Type Amino Acid Sequence Molecular Biology Cells Cultured Adipogenesis Activator (genetics) Binding protein Cell Biology Recombinant Proteins Cell biology 030104 developmental biology chemistry 030220 oncology & carcinogenesis Recombinant DNA Binding domain medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 519(3) |
| ISSN: | 1090-2104 |
| Popis: | Tetranectin (TN), a plasminogen (Plg) binding protein, enhances the Plg activator (PA)-catalyzed activation of Plg to plasmin (Pln). Previously, TN was identified as an adipogenic serum protein, which promotes adipocyte differentiation. In the present study, we investigated the adipogenic function of mouse TN using recombinant proteins (rmTNs) in full-length and domain-truncated forms. Adipocyte differentiation in TN-depleted-FBS-media was significantly enhanced by rmTNs supplementation. The adipogenic effect of rmTNs was found to be dependent on the presence of a Plg binding domain, indicating the domain is essential for the adipogenic function of mTN. In addition, these results suggested the involvement of Plg activation, however Plg, PA and Pln appeared to have no direct effect on adipocyte differentiation. This study demonstrates the adipogenic function of mTN, which is dependent on the Plg binding domain as its functional domain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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