Candida albicanssuppresses nitric oxide (NO) production by interferon-gamma (IFN-γ) and lipopolysaccharide (LPS)-stimulated murine peritoneal macrophages
| Autor: | T. Chinen, Kazuyoshi Kawakami, Yoshinobu Koguchi, Mahboob Hossain Qureshi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide medicine.medical_treatment Immunology In Vitro Techniques Nitric Oxide Nitric oxide Microbiology Interferon-gamma Mice chemistry.chemical_compound Immune system Neutralization Tests Transforming Growth Factor beta Candida albicans medicine Animals Immunology and Allergy Macrophage Interferon gamma Mice Inbred BALB C biology Antibodies Monoclonal Original Articles biology.organism_classification Recombinant Proteins In vitro Interleukin-10 Cytokine chemistry Macrophages Peritoneal Female Interleukin-1 medicine.drug |
| Zdroj: | Clinical and Experimental Immunology. 115:491-497 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.1999.00822.x |
| Popis: | SUMMARYWe examined the in vitro effect of Candida albicans on NO production by macrophages. Candida albicans suppressed not only NO production but also expression of inducible NO synthase (iNOS) mRNA by murine IFN-γ and bacterial LPS-stimulated peritoneal macrophages. The suppression was not associated with inhibition but rather stimulation of IL-1β production. This effect was observed when more than 1 × 103/ml of Candida albicans were added to macrophage cultures (1 × 106 cells/ml) and reached a maximal level at 1 × 106/ml. The NO inhibitory effect of Candida albicans was mediated predominantly by as yet unidentified soluble factor(s) and to a lesser extent by direct contact. In addition, heat- or paraformaldehyde-killed Candida albicans did not show this inhibitory activity. Culture supernatant of Candida albicans also inhibited NO production by activated macrophages in a dose-dependent manner, and increased IL-1β production. Finally, the inhibitory effect was not mediated by IL-10 and transforming growth factor-beta (TGF-β), since neutralizing antibodies to these cytokines did not influence Candida albicans-induced reduction in macrophage NO production. Our results suggest that Candida albicans may evade host defence mechanism(s) through a soluble factor-mediated suppression of NO production by stimulated macrophages, and that the effect is independent of production of immunosuppressive cytokines such as IL-10 and TGF-β. |
| Databáze: | OpenAIRE |
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