Role of CXC chemokine receptor-2 in a murine model of bronchopulmonary dysplasia

Autor: Erica Bäckström, Maija Bry, Heikki Lukkarinen, Anna Hogmalm, Kristina Bry, Urpo Lappalainen
Rok vydání: 2012
Předmět:
musculoskeletal diseases
Pulmonary and Respiratory Medicine
Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
Chemokine
Clinical Biochemistry
Interleukin-1beta
Gene Expression
Neovascularization
Physiologic
Inflammation
Apoptosis
Mice
Transgenic
Biology
Lung injury
Receptors
Interleukin-8B
Pathogenesis
Mice
Parenchyma
medicine
Morphogenesis
Animals
Calgranulin B
Humans
Calgranulin A
CXC chemokine receptors
Receptor
Molecular Biology
Lung
Bronchopulmonary Dysplasia
Cell Proliferation
Macrophages
Infant
Newborn
hemic and immune systems
Cell Biology
respiratory system
biological factors
respiratory tract diseases
Pulmonary Alveoli
Disease Models
Animal
medicine.anatomical_structure
Receptors
Vascular Endothelial Growth Factor
Matrix Metalloproteinase 9
Neutrophil Infiltration
Microvessels
biology.protein
medicine.symptom
Chemokines
CXC
Zdroj: American journal of respiratory cell and molecular biology. 47(6)
ISSN: 1535-4989
Popis: The contribution of neutrophils and CXC chemokines to the pathogenesis of bronchopulmonary dysplasia is not well defined. The transgenic expression of IL-1β in the pulmonary epithelium causes lung inflammation and disrupts alveolar development in infant mice. To study the hypothesis that CXC chemokine receptor-2 (CXCR2) is a mediator of inflammatory lung injury, we compared lung development in IL-1β-expressing mice with wild-type (IL-1β/CXCR2(+/+)) or null (IL-1β/CXCR2(-/-)) CXCR2 loci. CXCR2 deficiency abolished the transmigration of neutrophils into the alveolar lumen in IL-1β-expressing mice, but did not alter the number of neutrophils in the parenchyma. The deletion of CXCR2 increased the alveolar chord length and reduced the survival of mice when IL-1β was expressed from the pseudoglandular to the alveolar stages. The capillary configuration was highly abnormal in both IL-1β/CXCR2(+/+) and IL-1β/CXCR2(-/-) lungs, but in very different ways. The cellular area of the parenchyma and the total capillary area of IL-1β/CXCR2(+/+) and IL-1β/CXCR2(-/-) mice were smaller than those of control/CXCR2(+/+) and control/CXCR2(-/-) mice, but the ratio of capillary area to cellular area was similar in all four genotypes. When IL-1β was expressed during the saccular stage, IL-1β/CXCR2(-/-) mice had smaller alveolar chord lengths and better survival than did IL-1β/CXCR2(+/+) mice. Independent of the timing of IL-1β expression, IL-1β increased alveolar septal thickness in mice with wild-type CXCR2 loci, but not in CXCR2 null mice. Depending on the developmental stage at the time of the inflammatory insult, inhibition of the CXCR2 pathway may exert opposite effects on alveolar septation in the neonatal lung.
Databáze: OpenAIRE
Externí odkaz: