Ganciclovir-resistant cytomegalovirus clinical isolates: mode of resistance to ganciclovir
| Autor: | M C Jordan, Sylvia C. Stanat, J E Reardon, A Erice, W L Drew, Karen K. Biron |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
Ganciclovir Foscarnet DNA polymerase viruses Congenital cytomegalovirus infection Cytomegalovirus DNA-Directed DNA Polymerase Viral Plaque Assay medicine.disease_cause Antiviral Agents Virus Herpesviridae Microbiology stomatognathic system medicine Humans heterocyclic compounds Pharmacology (medical) Polymerase Vidarabine Nucleic Acid Synthesis Inhibitors Pharmacology Acquired Immunodeficiency Syndrome biology virus diseases Drug Resistance Microbial biochemical phenomena metabolism and nutrition medicine.disease Virology Infectious Diseases Cytomegalovirus Infections DNA Viral biology.protein Research Article medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 35:2191-2197 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.35.11.2191 |
| Popis: | Cytomegalovirus strains with reduced in vitro susceptibilities to ganciclovir have been recovered from patients who failed long-term ganciclovir therapy. The ganciclovir-resistant clinical isolates in this study were unable to induce ganciclovir phosphorylation in virus-infected cells. The viral DNA polymerase function appeared unaltered in one genetically pure ganciclovir-resistant strain, compared with that of its wild-type ganciclovir-sensitive counterpart. All nine of the ganciclovir-resistant strains were susceptible to foscarnet. Moreover, these strains were sensitive to inhibition both by vidarabine and 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-iodocytosine (FIAC), antiviral agents that are activated by cellular enzymes, and by (S)-1(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (HPMPC), which is a monophosphate nucleoside analog. The in vitro resistance to ganciclovir of the ganciclovir-resistant clinical isolates studied was attributed to the inability of the cells infected with these isolates to phosphorylate ganciclovir; the virally encoded DNA polymerase did not appear to play a role in this ganciclovir resistance. |
| Databáze: | OpenAIRE |
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