Characterization of the murine endothelial nitric oxide synthase promoter
| Autor: | Anouk-Martine Teichert, Kumudini Gnanapandithen, Xei Bei, Philip A. Marsden, Fotula Karantzoulis-Fegaras, Imtiaz A. Mawji, Yang Wang |
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| Rok vydání: | 1999 |
| Předmět: |
Endothelium
Nitric Oxide Synthase Type III Sequence analysis Recombinant Fusion Proteins Molecular Sequence Data Biophysics Nitric Oxide Synthase Type II Biology Regulatory Sequences Nucleic Acid Biochemistry Nitric oxide chemistry.chemical_compound Mice Structural Biology Enos Sequence Homology Nucleic Acid Genetic model Genetics medicine Animals Amino Acid Sequence Promoter Regions Genetic Gene Cells Cultured Regulation of gene expression Binding Sites Base Sequence DNA Sequence Analysis DNA biology.organism_classification Molecular biology medicine.anatomical_structure chemistry Cattle Endothelium Vascular Nitric Oxide Synthase Sequence Alignment Blood vessel Transcription Factors |
| Zdroj: | Biochimica et biophysica acta. 1443(3) |
| ISSN: | 0006-3002 |
| Popis: | As our understanding of the contributory roles of NO in the blood vessel wall evolves, so does the need to firmly understand the basic principles governing the regulated expression of the endothelial nitric oxide synthase (eNOS) gene. Because a robust approach to dissecting the relative contribution of a given cardiovascular gene exploits the use of murine genetic models, P1 murine genomic clones were isolated, characterized and functionally assessed to gain further insight into the regulated expression of the eNOS gene in the mouse. Sequence analysis of 1.8 kb of 5′ flanking regions revealed important regions of sequence conservation with human and bovine sequences. Functional promoter activity was confirmed using transient transfection analysis of cultured endothelial cells. |
| Databáze: | OpenAIRE |
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