Paired pulse plasticity in the strength and latency of light-evoked lateral inhibition to retinal bipolar cell terminals
| Autor: | Henrique von Gersdorff, Jozsef Vigh, Evan Vickers, Mean Hwan Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Retinal Bipolar Cells
Patch-Clamp Techniques Time Factors Light Pyridines Presynaptic Terminals Stimulation Tetrodotoxin Biology Plasticity In Vitro Techniques Inhibitory postsynaptic potential Article Biophysical Phenomena Retina GABA Antagonists chemistry.chemical_compound Lateral inhibition Goldfish Quinoxalines Neuroplasticity medicine Reaction Time Animals Drug Interactions Anesthetics Local Photons Neuronal Plasticity General Neuroscience Retinal Axotomy Neural Inhibition Inner plexiform layer Phosphinic Acids Electric Stimulation Pyridazines medicine.anatomical_structure chemistry Inhibitory Postsynaptic Potentials Neuroscience Excitatory Amino Acid Antagonists |
| Popis: | Synapses in the inner plexiform layer of the retina undergo short-term plasticity that may mediate different forms of adaptation to regularities in light stimuli. Using patch-clamp recordings from axotomized goldfish Mb bipolar cell (BC) terminals with paired-pulse light stimulation, we isolated and quantified the short-term plasticity of GABAergic lateral IPSCs (L-IPSCs). Bright light stimulation evoked ON and OFF L-IPSCs in axotomized BCs, which had distinct onset latencies (∼50–80 and ∼70–150 ms, respectively) that depended on background light adaptation. We observed plasticity in both the synaptic strength and latency of the L-IPSCs. With paired light stimulation, latencies of ON L-IPSCs increased at paired-pulse intervals (PPIs) of 50 and 300 ms, whereas OFF L-IPSC latencies decreased at the 300 ms PPI. ON L-IPSCs showed paired-pulse depression at intervals ARs and AMPARs differentially affected ON and OFF L-IPSCs, confirming that these two types of feedback inhibition are mediated by distinct and convergent retinal pathways with different mechanisms of plasticity. We propose that these plastic changes in the strength and timing of L-IPSCs help to dynamically shape the time course of glutamate release from ON-type BC terminals. Short-term plasticity of L-IPSCs may thus influence the strength, timing, and spatial extent of amacrine and ganglion cell inhibitory surrounds. |
| Databáze: | OpenAIRE |
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