Quantification of nitrogenous bases, DNA and Collagen type I for the estimation of the postmortem interval in bone remains
| Autor: | Cristina Pérez-Martínez, Aurelio Luna, Isabel Legaz, Gemma Prieto-Bonete, María D. Pérez-Cárceles |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Guanine Biology Bone and Bones Collagen Type I Mass Spectrometry Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Internal medicine medicine Humans 030216 legal & forensic medicine Purine metabolism Hypoxanthine Chromatography High Pressure Liquid Aged Aged 80 and over Discriminant Analysis DNA Middle Aged Xanthine Thymine 030104 developmental biology Endocrinology medicine.anatomical_structure Pyrimidines chemistry Biochemistry Purines Postmortem Changes Regression Analysis Cortical bone Female Law Cytosine Biomarkers |
| Zdroj: | Forensic science international. 281 |
| ISSN: | 1872-6283 |
| Popis: | Estimating the postmortem interval (PMI) is an important goal in forensic medicine and continues to be one of the most difficult tasks of the forensic investigator. Few accurate methods exist to determine the time since death of skeletonized human remains due to the great number of intrinsic and external factors that may alter the normal course of postmortem change. The purpose of this research was to assess the usefulness of various biochemical parameters, such as nitrogenous bases (adenine, guanine, purines, cytosine, thymine, pyrimidines, hypoxanthine and xanthine), DNA and Collagen Type I peptides to estimate PMI. These parameters were analysed in cortical bone for the establishment of data in a total of 80 long bones of 80 corpses (50 males, 30 females) with a mean age of 68.31 years (S.D.=18.021, range=20-97). The bones were removed from the cement niches of a cemetery in Murcia (south-eastern Spain), where they had lain for between 5 and 47 years (mean time 23.83 years, S.D.=10.85). Our results show a significant decrease in adenine (p=0.0004), guanine (p=0.0001), purines (p=0.0001), cytosine (p=0.0001), thymine (p=0.0226), pyrimidines (p=0.0002) and the number of peptides of Collagen type I (p=0.0053) in those with a PMI≥20 years. In a curvilinear regression analysis the results show that 30.6% of the variable PMI could be explained by guanine concentration, in bones with a PMI |
| Databáze: | OpenAIRE |
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