Nanostructured SBA-15 silica: An effective protective vehicle to oral hepatitis B vaccine immunization

Autor: Denise V. Tambourgi, Paulo R. A. F. Garcia, Gabriela D. Tanaka, Marcos Tadeu D’Azeredo Orlando, Joel José Megale Gabrili, Francisco Mariano Neto, Danielle Paixão-Cavalcante, Juliana Mussalem, Denise Cristina André Oliveira, Viviane Fongaro Botosso, Márcia Carvalho de Abreu Fantini, Osvaldo A. Sant'Anna, Karina Scaramuzzi, Cristiano L. P. Oliveira
Rok vydání: 2016
Předmět:
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1549-9634
DOI: 10.1016/j.nano.2016.06.003
Popis: Due to its physicochemical properties, nanostructured mesoporous SBA-15 silica shows great potential as a vaccine adjuvant. This study evaluated the capacity of SBA-15 to encapsulate/adsorb the recombinant purified HBsAg from the Hepatitis B virus and the immunoresponsiveness of mice orally immunized with HBsAg inside SBA-15. A simulation of small angle X-ray scattering experimental results, together with the nitrogen adsorption isotherms data, allowed to determine the appropriate mass ratio of HBsAg:SBA-15, indicating antigen encapsulation into SBA-15 macroporosity. This was also evaluated by bicinchoninic acid assay and gel electrophoresis. The recruitment of inflammatory cells, an increase in production of specific antibodies, and the non-influence of silica on TH1 or TH2 polarization were observed after oral immunization. Besides, SBA-15 enhanced the phagocytosis of ovalbumin by dendritic cells, an important key to prove how this adjuvant works. Thus, it seems clear that the nanostructured SBA-15 is an effective and safe adjuvant for oral immunizations.
Databáze: OpenAIRE
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