Hepatitis B virus and hepatitis delta virus subtypes circulating in Algeria and seroprevalence of HDV infection
| Autor: | Rim Khelifa, Hafida Djaballah, Paul Dény, Nabil Debzi, Abdelbasset Nani, Mohamed Tazir, Frédéric Le Gal, Mariama Abdou-Chekaraou, Ségolène Brichler, Nawel Afredj, Samir Gourari, Emmanuel Gordien, Mohamed Amine Beloufa, Mounira Boufekane |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Hepatitis B virus Adolescent Genotype Genotyping Techniques viruses Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Virus Serology Young Adult 03 medical and health sciences 0302 clinical medicine Seroepidemiologic Studies Virology medicine Humans Seroprevalence Hepatitis Antibodies 030212 general & internal medicine Child Genotyping Aged Molecular Epidemiology Molecular epidemiology virus diseases Sequence Analysis DNA Middle Aged biochemical phenomena metabolism and nutrition Hepatitis B Hepatitis D digestive system diseases Infectious Diseases Algeria Female 030211 gastroenterology & hepatology Hepatitis Delta Virus |
| Zdroj: | Journal of Medical Virology. 91:72-80 |
| ISSN: | 1096-9071 0146-6615 |
| Popis: | Background Little is known about molecular characteristics of HBV strains circulating in Algeria and there are few data regarding HDV infection. Objectives The aim of this study is to describe the genetic diversity of HBV and HDV strains existing in Algeria and to determine the seroprevalence of HDV infection. Study design Plasma samples from 134 patients were analyzed by enzyme immunoassay method for HBV and HDV serological markers. Genotyping of HBV and HDV strains were performed using direct sequencing followed by phylogenetic analyses of the PreS1 and R0 region of the HBV and HDV genome respectively. Results The PreS1 gene was successfully amplified in 119 patients (82 males and 37 females). Phylogenetic analysis of HBV strains revealed the presence of genotypes D (86.5%) and A2 (11.76%). The subgenotypes D are distributed as follows: HBV/D7 (43.5%), HBV/D3 (24.75%), HBV/D1 (16.8%) and HBV/D2 (14.85%). A recombinant between genotypes A, E and D was found. The seroprevalence of HDV infection among HBV carriers was less than 5.35%. Only one isolate of HDV genotype 1 was identified. Conclusions Our data indicate the predominance of HBV subgenotype D7 and a low prevalence of HDV infection. |
| Databáze: | OpenAIRE |
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