Systematic variation in gene expression patterns in human cancer cell lines
| Autor: | Douglas T. Ross, Alexander Pergamenschikov, Uwe Scherf, Christian A. Rees, Jeffrey C. Lee, Dari Shalon, David Botstein, Vishwanath R. Iyer, Stefanie S. Jeffrey, Charles M. Perou, Matt van de Rijn, John N. Weinstein, Michael B. Eisen, Deval A. Lashkari, Mark Waltham, Patrick O. Brown, Paul T. Spellman, Timothy G. Myers |
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| Rok vydání: | 2000 |
| Předmět: |
Stromal cell
DNA Complementary MDA-MB-435 Breast Neoplasms Biology medicine.disease_cause Neoplasms Gene expression Genetics medicine Tumor Cells Cultured Cluster Analysis Humans Breast Gene Oligonucleotide Array Sequence Analysis Regulation of gene expression Expressed Sequence Tags Leukemia Gene Expression Profiling Neoplasm Proteins Gene expression profiling Gene Expression Regulation Neoplastic Cell culture Organ Specificity Cancer research Female Carcinogenesis |
| Zdroj: | Nature genetics. 24(3) |
| ISSN: | 1061-4036 |
| Popis: | We used cDNA microarrays to explore the variation in expression of approximately 8,000 unique genes among the 60 cell lines used in the National Cancer Institute's screen for anti-cancer drugs. Classification of the cell lines based solely on the observed patterns of gene expression revealed a correspondence to the ostensible origins of the tumours from which the cell lines were derived. The consistent relationship between the gene expression patterns and the tissue of origin allowed us to recognize outliers whose previous classification appeared incorrect. Specific features of the gene expression patterns appeared to be related to physiological properties of the cell lines, such as their doubling time in culture, drug metabolism or the interferon response. Comparison of gene expression patterns in the cell lines to those observed in normal breast tissue or in breast tumour specimens revealed features of the expression patterns in the tumours that had recognizable counterparts in specific cell lines, reflecting the tumour, stromal and inflammatory components of the tumour tissue. These results provided a novel molecular characterization of this important group of human cell lines and their relationships to tumours in vivo. |
| Databáze: | OpenAIRE |
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