Chromosome analysis of blastocysts derived from single pronuclear zygotes by array CGH and clinical outcomes by the transfer of single pronuclear zygotes
| Autor: | Misato Taguchi, Akiko Ishiko, Yoko Izumi, Fuminori Kimura, Kimiko Hirata, Keiko Nakanishi, Sakae Goto, Takashi Murakami, Misono Fujioka, Ayano Hayashi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Zygote Aneuploidy Fertilization in Vitro Miscarriage Andrology 03 medical and health sciences 0302 clinical medicine Chromosome analysis Pregnancy Genetics medicine Single Embryo Transfer Humans Sperm Injections Intracytoplasmic Assisted Reproduction Technologies Genetics (clinical) reproductive and urinary physiology Chromosome Aberrations Cryopreservation Comparative Genomic Hybridization 030219 obstetrics & reproductive medicine business.industry urogenital system Blastocyst Transfer Pregnancy Outcome Obstetrics and Gynecology Small sample Embryo General Medicine medicine.disease 030104 developmental biology Blastocyst Reproductive Medicine embryonic structures Female business Developmental Biology |
| Zdroj: | J Assist Reprod Genet |
| ISSN: | 1573-7330 |
| Popis: | PURPOSE: This study aimed to explore the aneuploidy of blastocysts derived from single pronuclear (1PN) zygotes, almost 75% of which were regarded as diploid, using array CGH and examine the pregnancy outcomes. METHODS: Embryonic aneuploidy screening of sixteen embryos from 1PN zygotes and sixteen embryos from 2PN zygotes was performed using array CGH in study 1. In addition, the reproductive outcome of 1761 single blastocysts, after untested frozen-thawed blastocyst transfer in IVF/ICSI patients, was retrospectively analyzed and compared between the 1PN and 2PN groups in study 2. RESULTS: The aneuploidy rates were 30.8% (4/13) in 1PN IVF, 33.3% (1/3) in 1PN ICSI, 46.2% (6/13) in 2PN IVF, and 100% (3/3) in 2PN ICSI. The 1PN group achieved clinical pregnancy in 25.0% (7/28) of IVF and 30.0% (3/10) of ICSI, whereas these rates in the 2PN control group were 44.6% (557/1250) of IVF and 37.4% (177/473) of ICSI. No miscarriage occurred in the pregnancies from 1PN zygotes, whereas the rates of miscarriage in the 2PN control group were 22.6% (126/557) in IVF and 22.2% (39/176) in ICSI. The delivery rate was similar in all groups. Ten deliveries in the 1PN group showed no newborn malformation. CONCLUSION: Within the limits of the small sample size, our results suggest that the aneuploidy and delivery rates of the blastocysts derived from 1PN zygotes are the same as those derived from 2PN zygotes. Blastocysts derived from 1PN zygotes may be used clinically and could increase the chance of pregnancy. |
| Databáze: | OpenAIRE |
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