Multiresidue analysis of beta-agonists in bovine and porcine unrine, feed and hair using liquid chromatography electrospray ionisation tandem mass spectrometry
| Autor: | A.A.M. Stolker, Marieke B. Sanders, M.W.F. Nielen, J. E. Oosterink, J.J.P. Lasaroms, Thijs Meijer, T. Zuidema, Martien L. Essers |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Electrospray
Swine residue analysis Reproterol Mass spectrometry Tandem mass spectrometry liver Biochemistry Analytical Chemistry Theophylline Tandem Mass Spectrometry Terbutaline medicine Animals cleanup Solid phase extraction growth-promoting agents BU Microbiological & Chemical Food Analysis Chemical ionization Chromatography Molecular Structure Chemistry Selected reaction monitoring Organic Chemistry ms Adrenergic beta-Agonists Consumer protection Animal Feed Organische Chemie Drug Residues veterinary drugs Drug Combinations resonance Metaproterenol identification Cattle BU Microbiologische & Chemische Voedselanalyse Chromatography Liquid Hair medicine.drug |
| Zdroj: | Analytical and Bioanalytical Chemistry, 391(1), 199-210 Analytical and Bioanalytical Chemistry 391 (2008) 1 |
| ISSN: | 1618-2642 |
| Popis: | The use of beta-agonists as growth promoters in cattle breeding is forbidden in many countries for reasons of fair trade and consumer protection. In recent years the use of liquid chromatography (LC) tandem mass spectrometry (MS/MS) has been shown to be the method of choice for the control of beta-agonists. In this study an LC-MS/MS multiresidue analysis method is presented for trace analysis of 22 beta-agonists. A truly generic concept has been designed based on mixed-mode solid-phase extraction and positive electrospray ionisation LC-MS/MS operated in the multiple reaction monitoring mode. This method allows application to a wide variety of sample matrices such as urine, feed and hair, following minor modifications to the analysis procedure only. The method features fit-for-purpose sensitivity in urine as shown by CCalpha and CCbeta values of less than 0.2 and less than 0.5 microg/l respectively, for all beta-agonists studied (terbutaline and reproterol, less than 0.3 and less than 1.0 respectively). Similar but semiquantitative application to feed and hair showed CCbeta values of less than 10.0 and less than 5.0 microg/kg, respectively. A further simplification and improvement is demonstrated using Ultra Performance LC (UPLC) and fast-switching MS/MS. The successful validation of this method following the latest EU requirements and its application to real samples demonstrate that a new versatile tool has been achieved for veterinary control of beta-agonists. |
| Databáze: | OpenAIRE |
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