Two high-affinity interleukin 1 receptors represent separate gene products
| Autor: | David K. Lugg, Richard Anthony Chizzonite, Anne O. Chua, Perla Nunes, Ueli Gubler, Patricia L. Kilian, Kathryn P. Parker, K L Kaffka, Alvin S. Stern, Terri Truitt |
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| Rok vydání: | 1989 |
| Předmět: |
Transcription
Genetic Antigen presentation Receptors Antigen T-Cell Cell Line Interleukin 21 Mice Cytotoxic T cell Animals Humans IL-2 receptor RNA Messenger Receptors Immunologic Antigen-presenting cell Cells Cultured Interleukin 3 Multidisciplinary CD40 biology Cell Membrane Receptors Interleukin-1 Molecular biology Recombinant Proteins Cell biology Molecular Weight Kinetics Genes Interleukin 12 biology.protein Interleukin-1 Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 86(20) |
| ISSN: | 0027-8424 |
| Popis: | Interleukin 1 (IL-1) is a polypeptide hormone that mediates a broad range of biological activities and interacts with surface receptors on numerous cell types. Equilibrium binding studies have identified a class of IL-1 receptors on T cells, fibroblasts, and epithelial cells that have 2- to 5-fold higher affinity than the receptors on bone marrow cells, pre-B cells, and macrophage cell lines. Affinity cross-linking with human 125I-labeled IL-1 alpha (125I-IL-1 alpha) labels an approximately 100-kDa protein on T cells and fibroblasts and an approximately 80-kDa protein on pre-B cells and macrophage cell lines. Monoclonal and polyclonal antibodies specific for the IL-1 receptor on T cells and fibroblasts block human 125I-IL-1 alpha binding to T cells, fibroblasts, and epithelial cells but cannot block IL-1 binding to bone marrow cells, pre-B cells, and macrophages. These antibodies immunoprecipitate the IL-1 receptor-human 125I-IL-1 alpha complex from T cells and fibroblasts but not from pre-B cells and macrophage cell lines. An S1 nuclease protection assay demonstrated that T cells and fibroblasts contain identical IL-1 receptor mRNA but that pre-B cells and macrophages do not contain this receptor mRNA. Taken together, the data demonstrate that mouse T cells, fibroblasts, and epithelial cells express an identical IL-1 receptor, whereas the IL-1 receptor on pre-B cells, macrophages, and bone marrow cells represents a different gene product. |
| Databáze: | OpenAIRE |
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