Immuno-impedimetric Biosensor for Onsite Monitoring of Ascospores and Forecasting of Sclerotinia Stem Rot of Canola
| Autor: | Jie Chen, Zhimin Yan, Anh H. Nguyen, Jian Yang, Manzoor A. Shah, Scott MacKay, Gaser N. Abdelrasoul, Xiujie S. Li, Afreen Anwar, Lian C. T. Shoute, Mark T. McDermott, Donghai Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
Colony Count Microbial lcsh:Medicine Biosensing Techniques 02 engineering and technology Biology Plant Roots 01 natural sciences Article food Ascomycota Electric Impedance Canola lcsh:Science Plant Diseases Immunoassay Multidisciplinary Optical Imaging 010401 analytical chemistry Sclerotinia sclerotiorum lcsh:R Fungal pathogen Spores Fungal 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Ascocarp Horticulture Interdigitated electrode lcsh:Q Stem rot 0210 nano-technology Sclerotinia Biosensor |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Sclerotinia stem rot, caused by the fungal pathogen Sclerotinia sclerotiorum, is a destructive disease of canola and many other broadleaf crops. The primary inoculum responsible for initiating Sclerotinia epidemics is airborne ascospores released from the apothecia of sclerotia. Timely detection of the presence of airborne ascospores can serve as an early-warning system for forecasting and management of the disease. A major challenge is to develop a portable and automated device which can be deployed onsite to detect and quantify the presence of minute quantities of ascospores in the air and serves as a unit in a network of systems for forecasting of the epidemic. In this communication, we present the development of an impedimetric non-Faradaic biosensor based on anti-S. sclerotiorum polyclonal antibodies as probes to selectively capture the ascospores and sense their binding by an impedance based interdigitated electrode which was found to directly and unambiguously correlate the number of ascospores on sensor surface with the impedance response. |
| Databáze: | OpenAIRE |
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