| Popis: |
PDF file - 74KB, MLN4924 leads to downregulation of NFkappaB activity in a dose-dependent manner. (A) MLN4924 leads to downregulation of CXCR5 in CLL. CLL cells (N=9) were co-cultured with CD40L-expressing stroma for 24 hours, followed by incubation with 1 microM MLN4924 or vehicle control for 24 h. Total RNA was isolated from CD19+ CLL B-cells, reverse-transcribed and subjected to real-time PCR with the indicated probes (in duplicates). Results were normalized to 18S levels. (B-C) CLL cells (N=4) were co-cultured with CD40L-expressing stroma for 24 hours, followed by incubation with 0.25 or 1 microM MLN4924 or vehicle control for another 24 h. Nuclear protein fractions were isolated and p50 and p65 activity was measured by ELISA as per manufacturer's instructions (B). Total RNA was reverse-transcribed (C) and subjected to real-time PCR with the indicated probes (in duplicates). Results were normalized to 18S levels. Measurements were normalized to vehicle control and reported as a fold change. * - p |
