Potential of H1N1 influenza A virus as an air borne pathogen to induce infectivity in pancreas: a mouse model study

Autor:	Kaveh Sadeghi, Farhad Rezaei, Nastaran Ghavami, Vahid Salimi, Farid Azizi Jalilian, Talat Mokhtari Azad
Rok vydání:	2020
Předmět:	Environmental Engineering viruses Health Toxicology and Mutagenesis 02 engineering and technology 010501 environmental sciences Biology 01 natural sciences Applied Microbiology and Biotechnology Virus Immune system 020401 chemical engineering medicine 0204 chemical engineering Waste Management and Disposal Pathogen 0105 earth and related environmental sciences Water Science and Technology Infectivity Public Health Environmental and Occupational Health virus diseases medicine.disease Pollution Virology medicine.anatomical_structure Viral replication Cell culture Pancreatic injury Pancreas Research Article
Zdroj:	J Environ Health Sci Eng
ISSN:	2052-336X
Popis:	INTRODUCTION: H1N1 influenza virus, as an indoor/outdoor pathogen in air, can cause the flu-like illness and respiratory complication. The aim of this study was to evaluate the H1N1 influenza virus replication in pancreas and investigate the immune response against infected pancreas. MATERIAL AND METHODS: First, mouse pancreas cell line was infected by H1N1 influenza A virus using intranasally and intravenously infection methods, and then the pancreas tissue was collected and pathology experiment was carried out. Next, the protein and genome of influenza virus were detected using immunocytochemistry and real-time PCR, respectively. In addition, serum cytokines and serum lipase were investigated using ELISA. RESULT: The in-vitro results proved that the mouse pancreatic cell line can support influenza virus replication. The result also proved that influenza virus is capable to infect pancreas and induce pancreas damage. Further, the immune response in mice with infected pancreas exhibited a completely different pattern with that of mice infected through intranasal method. CONCLUSION: It can be concluded that influenza virus can infect pancreas and change the influenza disease pathway, which might result in a pancreatic injury.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::071d01dfbbfbf3e00418489fc67c01b5 https://doi.org/10.1007/s40201-020-00468-x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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