Superantigen gene profile diversity among clinical group A streptococcal isolates
| Autor: | Linda Maripuu, Mari Norgren, Anna Eriksson |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Streptococcus pyogenes Sequence analysis Bacterial Toxins Immunology Exotoxins Biology Polymerase Chain Reaction Microbiology DNA sequencing law.invention stomatognathic system law Streptococcal Infections Genetic variation Genotype Superantigen Humans Immunology and Allergy Gene Alleles Polymerase chain reaction Sweden Antigens Bacterial Superantigens Genetic Variation Sequence Analysis DNA General Medicine DNA Fingerprinting Infectious Diseases DNA profiling Carrier Proteins Bacterial Outer Membrane Proteins |
| Zdroj: | FEMS Immunology & Medical Microbiology. 54:236-244 |
| ISSN: | 1574-695X 0928-8244 |
| Popis: | This study examines the diversity of superantigen gene profiles between and within emm-genotypes of 92 clinical group A streptococcal isolates (30 STSS, 24 sepsis, 25 erysipelas, and 12 tonsillitis) collected in Sweden between 1986 and 2001. The emm-genotype and the distribution of smeZ, speG, speJ, speA, speC, speH, speI, speK/L, speL/M, speM, and ssa genes, and the smeZ allelic variant were determined using PCR and DNA sequencing. Forty-five emm1 isolates revealed 10 superantigen gene profiles. One profile dominated and was identified in 22 isolates collected over 14 years. The results indicate that a selective advantage maintained this genotype in circulation. The superantigen content among the emm1 isolates ranged from three to seven, with smeZ-1, speG, and speA present in all but one profile. The 47 isolates of 27 other emm-genotypes exhibited 29 superantigen gene profiles. Thus, the distribution of superantigen genes was highly variable within isolates regardless of emm-genotype. Two novel emm1 subtypes and 14 novel smeZ allelic variants were identified. The 22 smeZ alleles were generally linked to the emm-genotype. The results of the investigation show that superantigen gene profiling is useful for tracking spread of clones in the community. |
| Databáze: | OpenAIRE |
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