Fibroblast growth factor homologous factor 1 (FHF1) is expressed in a subpopulation of calcitonin gene-related peptide-positive nociceptive neurons in the murine dorsal root ganglia

Autor: Jean Valmier, Stéphanie Ventéo, Patrick Carroll, Agnès Fichard-Carroll, Sylvie Puech, Ilana Mechaly, Thomas Hubert, Steeve Bourane
Přispěvatelé: Hamel, Christian, Physiopathologie et thérapie des déficits sensoriels et moteurs, Université Montpellier 2 - Sciences et Techniques (UM2)-IFR76-Institut National de la Santé et de la Recherche Médicale (INSERM)
Rok vydání: 2008
Předmět:
MESH: Neurons
Fluorescent Antibody Technique
MESH: Neuropeptides
Fibroblast growth factor
Sodium Channels
Mice
Neurotrophic factors
MESH: Reverse Transcriptase Polymerase Chain Reaction
Ganglia
Spinal
MESH: Animals
MESH: Nociceptors
MESH: Fluorescent Antibody Technique
In Situ Hybridization
Neurons
Sodium channel activity
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
MESH: Receptor
trkA
Nociceptors
Axotomy
Peripherin
Immunohistochemistry
Cell biology
Nociception
MESH: Ganglia
Spinal
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
MESH: Fibroblast Growth Factors
Neurofilament
Calcitonin Gene-Related Peptide
Blotting
Western
MESH: Axotomy
Calcitonin gene-related peptide
Biology
MESH: Sodium Channels
MESH: In Situ Hybridization
MESH: Mice
Inbred C57BL
MESH: Blotting
Western
MESH: Calcitonin Gene-Related Peptide
Animals
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
RNA
Messenger
Receptor
trkA
MESH: Mice
NAV1.9 Voltage-Gated Sodium Channel
MESH: RNA
Messenger
Sodium channel
Neuropeptides
MESH: Immunohistochemistry
Fibroblast Growth Factors
Mice
Inbred C57BL
nervous system
Neuroscience
Zdroj: Journal of Comparative Neurology
Journal of Comparative Neurology, Wiley, 2008, 507 (4), pp.1588-601. ⟨10.1002/cne.21631⟩
ISSN: 1096-9861
0021-9967
DOI: 10.1002/cne.21631
Popis: International audience; Dorsal root ganglia (DRG) neurons exhibit a wide molecular heterogeneity in relation to the various sensory modalities (mechanoception, thermoception, nociception) that they subserve. Finding markers of subpopulations is an important step in understanding how these neurons convey specific information. We identified fibroblast growth factor homologous factor 1 (FHF1) in a search for markers of subpopulations of DRG neurons. FHFs constitute a family of four factors that share some structural properties with fibroblast growth factors (FGFs) but are functionally distinct. They are expressed in specific subsets of neurons and are involved in the modulation of sodium channel activity. The pattern of expression of FHF1 in the DRG was determined during development, in the adult and after axotomy. We show that in the adult, FHF1 is expressed in two populations, one composed of nociceptors and another in which no neurotrophic factor receptors were detected (panTrk-/c-Ret-). Interestingly, in the nociceptors, FHF1 expression was restricted to a subset of TrkA+/calcitonin gene-related peptide (CGRP)-positive neurons. Neurofilament 200 (NF-200) and peripherin labeling indicates that 70% of the FHF1-expressing neurons contribute to A-fibers and 30% to C-fibers. FHF1 interacts with the Na(v)1.9 sodium channel isoform, which is strongly expressed in cRet+/isolectin-B4 binding neurons, but we show that FHF1 is not expressed in the cRet+/IB4+ subclass and that it does not colocalize with Na(v)1.9. Our results argue strongly against the possibility that FHF1 has a modulatory effect on this channel in cRet+/IB4+ neurons, but FHF1 could play a role in a distinct subset of TrkA+/CGRP+ nociceptors.
Databáze: OpenAIRE
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