Piper sarmentosum Roxb. produces antidepressant-like effects in rodents, associated with activation of the CREB-BDNF-ERK signaling pathway and reversal of HPA axis hyperactivity
Autor: | Kuo Hsiung Lee, Khalid Rahman, Ting Han, Fa Lin Qu, Yi-Ping Jiang, Lu-Ping Qin, Yue Gao, Qing Li |
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Rok vydání: | 2017 |
Předmět: |
Male
0301 basic medicine Hypothalamo-Hypophyseal System RM medicine.medical_specialty MAP Kinase Signaling System Pituitary-Adrenal System RV Piper sarmentosum Pharmacology CREB Open field Rats Sprague-Dawley Mice Random Allocation 03 medical and health sciences 0302 clinical medicine Western blot Neurotrophic factors Internal medicine Drug Discovery medicine Animals Hippocampus (mythology) Cyclic AMP Response Element-Binding Protein Mice Inbred ICR Dose-Response Relationship Drug biology medicine.diagnostic_test Depression Plant Extracts business.industry Brain-Derived Neurotrophic Factor Plant Components Aerial biology.organism_classification Antidepressive Agents Tail suspension test Rats 030104 developmental biology Endocrinology biology.protein business Piper 030217 neurology & neurosurgery Behavioural despair test |
Zdroj: | Journal of Ethnopharmacology. 199:9-19 |
ISSN: | 0378-8741 1872-7573 |
Popis: | ETHNOPHARMACOLOGICAL RELEVANCE: There are many plants of genus Piper which have been reported to induce antidepressant-like effects, Piper sarmentosum (PS) is one of them. PS is a Chinese herbal medicine and a traditional edible vegetable. MATERIALS AND METHODS: In the present study, the antidepressant-like effects of PS extracts and the ethyl acetate fraction of PS extracts (PSY) were assessed using the open field test (OFT), forced swimming test (FST), and tail suspension test (TST) in mice. Furthermore, we applied a 4 consecutive weeks of chronic unpredictable mild stress (CUMS) as a model of depression in rats, followed by a sucrose preference test. Then we examined the possible mechanisms of this action. The activity of the hypothalamic-pituitary-adrenal (HPA) axis was evaluated by detecting the serum corticosterone (CORT) concentrations, and the protein expression levels of brain-derived neurotrophic factor (BDNF), the phosphorylated form CREB and ERK1/2 were detected by qRT-PCR or Western blot. RESULTS: The results showed that PS extracts (100, 200mg/kg) and PSY (12.5, 25, 50mg/kg) treatment produced antidepressant-like effects in mice similar to fluoxetine (20mg/kg), indicated by the reduced immobility time in the FST and TST, while both had no influence on the locomotor activity in the OFT. PSY treatment significantly increased sucrose preference and reduced serum CORT levels in CUMS rats. Moreover, PSY up-regulated BDNF protein levels, and increased CREB and ERK phosphorylation levels in the hippocampus on CUMS rats. CONCLUSIONS: These findings suggest that the antidepressant-like effects of PS extracts and PSY are mediated, at least in part, by modulating HPA axis, BDNF, CREB and ERK phosphorylation and expression in the hippocampus. |
Databáze: | OpenAIRE |
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