Piper sarmentosum Roxb. produces antidepressant-like effects in rodents, associated with activation of the CREB-BDNF-ERK signaling pathway and reversal of HPA axis hyperactivity

Autor: Kuo Hsiung Lee, Khalid Rahman, Ting Han, Fa Lin Qu, Yi-Ping Jiang, Lu-Ping Qin, Yue Gao, Qing Li
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Hypothalamo-Hypophyseal System
RM
medicine.medical_specialty
MAP Kinase Signaling System
Pituitary-Adrenal System
RV
Piper sarmentosum
Pharmacology
CREB
Open field
Rats
Sprague-Dawley
Mice
Random Allocation
03 medical and health sciences
0302 clinical medicine
Western blot
Neurotrophic factors
Internal medicine
Drug Discovery
medicine
Animals
Hippocampus (mythology)
Cyclic AMP Response Element-Binding Protein
Mice
Inbred ICR
Dose-Response Relationship
Drug
biology
medicine.diagnostic_test
Depression
Plant Extracts
business.industry
Brain-Derived Neurotrophic Factor
Plant Components
Aerial
biology.organism_classification
Antidepressive Agents
Tail suspension test
Rats
030104 developmental biology
Endocrinology
biology.protein
business
Piper
030217 neurology & neurosurgery
Behavioural despair test
Zdroj: Journal of Ethnopharmacology. 199:9-19
ISSN: 0378-8741
1872-7573
Popis: ETHNOPHARMACOLOGICAL RELEVANCE: There are many plants of genus Piper which have been reported to induce antidepressant-like effects, Piper sarmentosum (PS) is one of them. PS is a Chinese herbal medicine and a traditional edible vegetable. MATERIALS AND METHODS: In the present study, the antidepressant-like effects of PS extracts and the ethyl acetate fraction of PS extracts (PSY) were assessed using the open field test (OFT), forced swimming test (FST), and tail suspension test (TST) in mice. Furthermore, we applied a 4 consecutive weeks of chronic unpredictable mild stress (CUMS) as a model of depression in rats, followed by a sucrose preference test. Then we examined the possible mechanisms of this action. The activity of the hypothalamic-pituitary-adrenal (HPA) axis was evaluated by detecting the serum corticosterone (CORT) concentrations, and the protein expression levels of brain-derived neurotrophic factor (BDNF), the phosphorylated form CREB and ERK1/2 were detected by qRT-PCR or Western blot. RESULTS: The results showed that PS extracts (100, 200mg/kg) and PSY (12.5, 25, 50mg/kg) treatment produced antidepressant-like effects in mice similar to fluoxetine (20mg/kg), indicated by the reduced immobility time in the FST and TST, while both had no influence on the locomotor activity in the OFT. PSY treatment significantly increased sucrose preference and reduced serum CORT levels in CUMS rats. Moreover, PSY up-regulated BDNF protein levels, and increased CREB and ERK phosphorylation levels in the hippocampus on CUMS rats. CONCLUSIONS: These findings suggest that the antidepressant-like effects of PS extracts and PSY are mediated, at least in part, by modulating HPA axis, BDNF, CREB and ERK phosphorylation and expression in the hippocampus.
Databáze: OpenAIRE
Externí odkaz: