Molecular detection of Neospora caninum from naturally infected four passeriforme birds in China
| Autor: | Bin-Ze Gui, Wen-Bin Zheng, Qiu-Yan Lv, Xing-Quan Zhu, Meng-Ting Liu, Jianhua Li, Wei-Xing Jiang, Guo-Hua Liu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
China Veterinary (miscellaneous) 030231 tropical medicine Bunting Anthus hodgsoni Zoology Animals Wild Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Emberiza rutila parasitic diseases Animals Parasite hosting Passeriformes Internal transcribed spacer Ribosomal DNA Phylogeny biology Coccidiosis fungi Neospora DNA Protozoan 030108 mycology & parasitology biology.organism_classification Neospora caninum Infectious Diseases Insect Science Parasitology Nested polymerase chain reaction |
| Zdroj: | Acta Tropica. 197:105044 |
| ISSN: | 0001-706X |
| DOI: | 10.1016/j.actatropica.2019.105044 |
| Popis: | Neospora caninum is an intracellular protozoan infecting many domestic and wild animals. In the present study, the brain tissues of wild birds collected in Hunan province of China were examined by N. caninum specific nested PCR, targeting the Nc-5 gene and the internal transcribed spacer 1 (ITS-1) region of the nuclear ribosomal DNA. The prevalence of N. caninum was detected in 15.5% (37/239) of wild birds, including 20.5% (9/44) of the examined olive-backed pipit Anthus hodgsoni, 18.3% (24/131) of the examined tree sparrows Passer montanus, 7.9% (3/38) of the examined chestnut bunting Emberiza rutila and 3.8% (1/26) of the examined yellow-breasted bunting E. aureola. Phylogenetic analyses showed that N. caninum from different hosts and geographical origins are genetically diverse and can be further classified into two distinct groups. Our findings indicated that wild birds are potential source of N. caninum for other animals. To our knowledge, this is the first report of N. caninum infection in wild birds in China, which provides a foundation for the prevention and control of this parasite in China and elsewhere. |
| Databáze: | OpenAIRE |
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